Recombinant Human Neuroserpin protein (ab63224)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesHuman
    • SequenceMTGATFPEEA IADLSVNMYN RLRATGEDEN ILFSPLSIAL AMGMMELGAQ GSTQKEIRHS MGYDSLKNGE EFSFLKEFSN MVTAKESQYV MKIANSLFVQ NGFHVNEEFL QMMKKYFNAA VNHVDFSQNV AVANYINKWV ENNTNNLVKD LVSPRDFDAA TYLALINAVY FKGNWKSQFR PENTRTFSFT KDDESEVQIP MMYQQGEFYY GEFSDGSNEA GGIYQVLEIP YEGDEISMML VLSRQEVPLA TLEPLVKAQL VEEWANSVKK QKVEVYLPRF TVEQEIDLKD VLKALGITEI FIKDANLTGL SDNKEIFLSK AIHKSFLEVN EEGSEAAAVS GMIAISRMAV LYPQVIVDHP FFFLIRNRRT GTILFMGRVM HPETMNTSGH DFEEL

Specifications

Our Abpromise guarantee covers the use of ab63224 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

    HPLC

    Western blot

  • Endotoxin level< 0.100 Eu/µg
  • Purity> 95 % SDS-PAGE.
    Greater than 98% by SDS-PAGE gel and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg).
  • FormLyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

General Info

  • Alternative names
    • DKFZp781N13156
    • Neuroserpin
    • NEUS_HUMAN
    • Peptidase inhibitor 12
    • PI-12
    • PI12
    • Protease inhibitor 12
    • Serine or cysteine proteinase inhibitor clade I (neuroserpin) member 1
    • Serine or cysteine proteinase inhibitor clade I member 1
    • Serpin I1
    • Serpin peptidase inhibitor clade I (neuroserpin) member 1
    • SERPINI1
    see all
  • FunctionSerine protease inhibitor that inhibits plasminogen activators and plasmin but not thrombin. May be involved in the formation or reorganization of synaptic connections as well as for synaptic plasticity in the adult nervous system. May protect neurons from cell damage by tissue-type plasminogen activator.
  • Tissue specificityPredominantly expressed in the brain.
  • Involvement in diseaseDefects in SERPINI1 are the cause of familial encephalopathy with neuroserpin inclusion bodies (FEN1B) [MIM:604218]. FEN1B is characterized clinically as an autosomal dominantly inherited dementia, histologically by unique neuronal inclusion bodies and biochemically by polymers of neuroserpin.
  • Sequence similaritiesBelongs to the serpin family.
  • Cellular localizationSecreted.
  • Information by UniProt

Recombinant Human Neuroserpin protein images

  • Anti-Neuroserpin antibody (ab16171) at 1/2500 dilution + Recombinant Human Neuroserpin protein (ab63224) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds

References for Recombinant Human Neuroserpin protein (ab63224)

This product has been referenced in:
  • Subhadra B  et al. Neuroserpin up-regulation in the Alzheimer's disease brain is associated with elevated thyroid hormone receptor-ß1 and HuD expression. Neurochem Int 63:476-81 (2013). Read more (PubMed: 24036060) »

See 1 Publication for this product

Product Wall

Thank you for sending that information.

I have gone ahead and uploaded all of that information into the Abreview and have also published the Abreview.

Please let me know if there is anything else I can help you with.

Application Other

Review text: Comparing the tPA inhibitory activity of Abcam Neuroserpin versus mouse neuroserpin

Procedure: tPA proteolytic assay using

Results:
Mouse neuroserpin inhibited ~70% tPA activity in 5 minutes where as Abcam neuroserpin inhibited ~20 % of tPA activity in 5 minutes. A 1:100 molar ratio of
Abcam NS to tPA gave no better inhibition, if anything it was less inhibitory.
Sample: Escherichia coli

Primary antibody (in addition to 'Neuroserpin protein')
Primary antibody: None used

Secondary antibody
Secondary antibody: None used
Username

Abcam user community

Verified customer

Submitted Mar 07 2012

DISCOUNT CODE: ************ Expiration date:************* I am very pleased to hear you would like to use ab63224 in an Inhibition Assayand send us an image of your results. This code will give you 1 free PROTEIN before the expiration date. To redeem t...

Read More

Thank you for contacting Abcam. We have not tested ab63224 to see if it inhibits TPA activity. We do not have sample sizes of our products, but what I can offer is out testing disount program. Asab63224 has not been tested in an inhibition assay, I can...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"