Recombinant human NFkB Inducing Kinase NIK protein (ab105210)

Overview

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • AccessionQ99558
    • SpeciesHuman
    • Molecular weight108 kDa including tags
    • Amino acids325 to 947

Associated products

Specifications

Our Abpromise guarantee covers the use of ab105210 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityThe Specific activity of ab105210 was determined to be 8 nmol/min/mg.
  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

  • Purity> 85 % SDS-PAGE.
    Purity was determined to be >85% by densitometry.
  • FormLiquid
  • Additional notes

    ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: 0% None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • MAP3K14
    • FTDCR1B
    • HS
    • HsNIK
    • M3K14_HUMAN
    • Map3k14
    • Mitogen activated protein kinase kinase kinase 14
    • Mitogen-activated protein kinase kinase kinase 14
    • NF kappa beta inducing kinase
    • NF-kappa-beta-inducing kinase
    • NIK
    • Serine/threonine-protein kinase NIK
    see all
  • FunctionLymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Promotes proteolytic processing of NFKB2/P100, which leads to activation of NF-kappa-B via the non-canonical pathway. Could act in a receptor-selective manner.
  • Tissue specificityWeakly expressed in testis, small intestine, spleen, thymus, peripheral blood leukocytes, prostate, ovary and colon.
  • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated. Phosphorylation at Thr-559 is required to activates its kinase activity and 'Lys-63'-linked polyubiquitination. Phosphorylated by CHUK/IKKA leading to MAP3K14 destabilization.
    Ubiquitinated. Undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. 'Lys-48'-linked polyubiquitination leads to its degradation by the proteasome, while 'Lys-63'-linked polyubiquitination stabilizes and activates it.
  • Cellular localizationCytoplasm.
  • Information by UniProt

Recombinant human NFkB Inducing Kinase NIK protein images

  • The Specific activity of ab105210 was determined to be 8 nmol/min/mg.
  • SDS-PAGE showing ab105210 at approximately 108kDa.

References for Recombinant human NFkB Inducing Kinase NIK protein (ab105210)

ab105210 has not yet been referenced specifically in any publications.

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