Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionQ9NX24
    • SpeciesHuman
    • SequenceMGSSHHHHHHSSGLVPRGSHMTKIKADPDGPEAQAEACSGERTYQELLVN QNPIAQPLASRRLTRKLYKCIKKAVKQKQIRRGVKEVQKFVNKGEKGIMV LAGDTLPIEVYCHLPVMCEDRNLPYVYIPSKTDLGAAAGSKRPTCVIMVK PHEEYQEAYDECLEEVQSLPLPL
    • Molecular weight19 kDa including tags
    • Amino acids1 to 153
    • TagsHis tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab102027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry
    MALDI-TOF
  • Purity> 90 % SDS-PAGE.
    ab102027 was purified using conventional chromatography.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 20% Glycerol, 0.1M Sodium chloride, 20mM Tris HCl, 1mM DTT, pH 8.0

General Info

  • Alternative names
    • DKCB2
    • FLJ20479
    • H/ACA ribonucleoprotein complex subunit 2
    • NHP2
    • NHP2 like protein
    • NHP2 ribonucleoprotein
    • NHP2 ribonucleoprotein homolog
    • NHP2 ribonucleoprotein homolog (yeast) 1
    • NHP2, S. cerevisiae, homolog of
    • NHP2_HUMAN
    • NHP2P
    • NOLA2
    • Nucleolar protein family A member 2
    • Nucleolar protein family A member 2 (H/ACA small nucleolar RNPs)
    • snoRNP protein NHP2
    see all
  • FunctionRequired for ribosome biogenesis and telomere maintenance. Part of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ("psi") residues, which may serve to stabilize the conformation of rRNAs. May also be required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme.
  • Tissue specificityExpressed in brain, colon, heart, kidney, ovary, pancreas, placenta, prostate, skeletal muscle, small intestine, spleen, testis and thymus. Also expressed at lower levels in the liver.
  • Involvement in diseaseDyskeratosis congenita, autosomal recessive, 2
  • Sequence similaritiesBelongs to the ribosomal protein L7Ae family.
  • Developmental stageTranscript peaks at G1/S transition.
  • Cellular localizationNucleus > nucleolus. Nucleus > Cajal body. Also localized to Cajal bodies.
  • Information by UniProt

Recombinant Human NHP2 protein images

  • 15% SDS-PAGE analysis of ab102027 (3µg)

References for Recombinant Human NHP2 protein (ab102027)

ab102027 has not yet been referenced specifically in any publications.

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