Overview

Description

  • NatureRecombinant
  • SourceWheat germ
  • Amino Acid Sequence
    • AccessionQ12972
    • SpeciesHuman
    • SequenceRRMQNFAFSGGLYGGLPPTHSEAGSQPHGIHGTALIGGLPMPYPNLAPDV DLTPVVPSAVNMNPAPNPAVYNPEAVNEPKKKKYAKEAWPGKKPTPSLLI
    • Molecular weight37 kDa including tags
    • Amino acids28 to 127

Specifications

Our Abpromise guarantee covers the use of ab114902 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

    ELISA

  • FormLiquid
  • Additional notesProtein concentration is above or equal to 0.05 mg/ml. Best use within three months from the date of receipt of this protein.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.3% Glutathione, 0.79% Tris HCl

General Info

  • Alternative names
    • Activator of RNA decay
    • ARD 1
    • ARD-1
    • ARD1
    • Homolog of E.coli RNase E
    • NIPP 1
    • NIPP-1
    • NIPP1
    • Nuclear inhibitor of protein phosphatase 1
    • nuclear inhibitor of protein phosphatase-1 alpha
    • Nuclear subunit of PP1
    • PP1R8_HUMAN
    • PPP1R8
    • PRO2047
    • Protein phosphatase 1 regulatory inhibitor subunit 8
    • Protein phosphatase 1 regulatory subunit 8
    • RNase E
    • RNase E, E. coli, homolog of
    see all
  • FunctionInhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. May also be involved in pre-mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation.
    Isoform Gamma is a site-specific single-strand endoribonuclease that cleaves single strand RNA 3' to purines and pyrimidines in A+U-rich regions. It generates 5'-phosphate termini at the site of cleavage. This isoform does not inhibit PP-1. May be implicated in mRNA splicing.
  • Tissue specificityUbiquitously expressed, with highest levels in heart and skeletal muscle, followed by brain, placenta, lung, liver and pancreas. Less abundant in kidney. The concentration and ratio between isoforms is cell-type dependent. Isoform Alpha (>90%) and isoform Beta were found in brain, heart and kidney. Isoform Gamma is mainly found in B-cells and T-lymphocytes, and has been found in 293 embryonic kidney cells.
  • Sequence similaritiesContains 1 FHA domain.
  • DomainHas a basic N- and C-terminal and an acidic central domain.
  • Post-translational
    modifications
    May be inactivated by phosphorylation on Ser-199 or Ser-204 (By similarity). Phosphorylated by Lyn in vitro on Tyr-264, and also on Tyr-335 in the presence of RNA.
  • Cellular localizationNucleus. Nucleus speckle. Primarily, but not exclusively, nuclear and Cytoplasm. Found mainly in the cytoplasm.
  • Information by UniProt

Recombinant Human NIPP1 protein images

  • 12.5% SDS-PAGE showing ab114902 at approximately 36.63kDa.
    Stained with Coomassie Blue.

References for Recombinant Human NIPP1 protein (ab114902)

ab114902 has not yet been referenced specifically in any publications.

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