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Baculovirus infected Sf9 cells
Amino Acid Sequence
KGAVDDAIAFGGKTDQEAPNASQPTPPPLPKKMIIRANTEPISKDLQKSM ESSLCVMANPTYDIDPNWDASSAGSSISYELKGLDIESYDSLERPLRKER PVPSAANSISSLTTLSIKDRFSNSMESLSSRRGPSCRQGRGIQKPQRQAL YRGLENREEVVGKIRSLHTDALKKLAVKCEDLFMAGQKDQLRFGVDSWSD FRLTSDKPCCEAGDAVYYTASYAKDPLNNYAVKICKSKAKESQQYYHSLA VRQSLAVHFNIQQDCGHFLAEVPNRLLPWEDPDDPEKDEDDMEETEEDAK GETDGKNPKPCSEAASSQKENQGVMSKKQRSHVVVITREVPCLTVADFVR DSLAQHGKSPDLYERQVCLLLLQLCSGLEHLKPYHVTHCDLRLENLLLVH YQPGGTAQGFGPAEPSPTSSYPTRLIVSNFSQAKQKSHLVDPEILRDQSR LAPEIITATQYKKCDEFQTGILIYEMLHLPNPFDENPELKEREYTRADLP RIPFRSPYSRGLQQLASCLLNPNPSERILISDAKGILQCLLWGPREDLFQ TFTACPSLVQRNTLLQNWLDIKRTLLMIKFAEKSLDREGGISLEDWLCAQ YLAFATTDSLSCIVKILQHR
100 kDa including tags
1127 to 1746
proprietary tag N-Terminus
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
It is recommended to store this protein at -80°C in glycerol to preserve the activity of the protein.
ab204877 (Poly (4:1 Glu, Tyr) peptide) can be utilized as a substrate for assessing kinase activity
Concentration information loading...
Stability and Storage
Shipped on Dry Ice. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Tyrosine kinase that may play a role in cell spreading and migration on fibronectin. May directly or indirectly affect phosphorylation levels of cytoskeleton-associated proteins MAPK1/ERK and PXN.
Belongs to the protein kinase superfamily.
Contains 1 protein kinase domain.
Phosphorylated on tyrosine in a CSK-dependent manner in response to adhesion to fibronectin and to EGF stimulation. Autophosphorylated in vitro.
Cytoplasm > cytoskeleton. Cell junction > focal adhesion. Colocalizes with F-actin in serum-rich medium. Actin colocalization is reduced during serum starvation.
Information by UniProt
Functional Studies - Active human NKF3 kinase family member protein fragment (ab177257)
The specific activity of ab177257 was determined to be 6 nmol/min/mg.
SDS-PAGE - Active human NKF3 kinase family member protein fragment (ab177257)
SDS-PAGE analysis of ab177257.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"