Overview

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • AccessionQ9UBE8
    • SpeciesHuman
    • Molecular weight84 kDa including tags
    • Amino acids1 to 527

Specifications

Our Abpromise guarantee covers the use of ab107700 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activitySpecific activity: 2.4 nmol/min/mg
  • Applications

    SDS-PAGE

    Western blot

    Functional Studies

  • Purity> 85 % SDS-PAGE.
    The purity was determined to be >85% by densitometry
  • FormLiquid
  • Additional notes

    ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • DKFZp761G1211
    • FLJ21033
    • LAK1
    • Nemo like kinase
    • Nemo-like kinase
    • Nlk
    • NLK_HUMAN
    • Protein LAK1
    • Serine/threonine protein kinase NLK
    • Serine/threonine-protein kinase NLK
    see all
  • FunctionRole in cell fate determination, required for differentiation of bone marrow stromal cells. Acts downstream of MAP3K7 and HIPK2 to negatively regulate the canonical Wnt/beta-catenin signaling pathway and the phosphorylation and destruction of the MYB transcription factor. May suppress a wide range of transcription factors by phosphorylation of the coactivator, CREBBP (By similarity). Involved in TGFbeta-mediated mesoderm induction, acting downstream of MAP3K7/TAK1 to phosphorylate STAT3.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Dually phosphorylated on Thr-303 and Tyr-305, which activates the enzyme.
  • Cellular localizationNucleus. Cytoplasm. Predominantly nuclear. A smaller fraction is cytoplasmic.
  • Information by UniProt

Recombinant human NLK protein images

  • SDS-PAGE analysis of ab107700
  • Specific activity of ab107700 was determined to be 2.4 nmol/min/mg.

References for Recombinant human NLK protein (ab107700)

ab107700 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"