Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesHuman
    • SequenceMANLERTFIA IKPDGVQRGL VGEIIKRFEQ KGFRLVAMKF LRASEEHLKQ HYIDLKDRPF FPGLVKYMNS GPVVAMVWEG LNVVKTGRVM LGETNPADSK PGTIRGDFCI QVGRNIIHGS DSVKSAEKEI SLWFKPEELV DYKSCAHDWV YE

Specifications

Our Abpromise guarantee covers the use of ab93692 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

  • Purity> 95 % SDS-PAGE.
    ab93692 is purified using conventional chromatography techniques.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Tris HCl, 1mM DTT, pH 8.0

General Info

  • Alternative names
    • C myc purine binding transcription factor PUF
    • C myc transcription factor
    • C-myc purine-binding transcription factor PUF
    • epididymis secretory sperm binding protein Li 155an
    • HEL-S-155an
    • Histidine protein kinase NDKB
    • MGC111212
    • MGC2212
    • NDK B
    • NDKB
    • NDKB_HUMAN
    • NDP kinase B
    • NDPK B
    • NDPKB
    • NM23 H2
    • nm23-H2
    • NM23B
    • NME/NM23 nucleoside diphosphate kinase 2
    • nme2
    • Non metastatic cells 2, protein (NM23B) expressed in
    • non-metastatic cells 2, protein (NM23) expressed in
    • Nucleoside diphosphate kinase B
    • Nucleotide diphosphate kinase B
    • PUF
    see all
  • FunctionMajor role in the synthesis of nucleoside triphosphates other than ATP. Negatively regulates Rho activity by interacting with AKAP13/LBC. Acts as a transcriptional activator of the MYC gene; binds DNA non-specifically (PubMed:8392752). Exhibits histidine protein kinase activity.
  • Tissue specificityUbiquitously expressed.
  • Sequence similaritiesBelongs to the NDK family.
  • Cellular localizationCytoplasm. Nucleus. Isoform 2 is mainly cytoplasmic and isoform 1 and isoform 2 are excluded from the nucleolus.
  • Information by UniProt

Recombinant Human NME2 protein images

  • 15% SDS-PAGE showing ab93692 at approximately 17 kDa (3 µg).
  • Anti-NME2 antibody (ab60602) at 1/1000 dilution + Recombinant Human NME2 protein (ab93692) at 0.1 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 2 minutes

References for Recombinant Human NME2 protein (ab93692)

ab93692 has not yet been referenced specifically in any publications.

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