Recombinant human NMNAT3 protein (ab157274)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MKSRIPVVLLACGSFNPITNMHLRMFEVARDHLHQTGMYQVIQGIISPVN DTYGKKDLAASHHRVAMARLALQTSDWIRVDPWESEQAQWMETVKVLRHH HSKLLRSPPQMEGPDHGKALFSTPAAVPELKLLCGADVLKTFQTPNLWKD AHIQEIVEKFGLVCVGRVGHDPKGYIAESPILRMHQHNIHLAKEPVQNEI SATYIRRALGQGQSVKYLIPDAVITYIKDHGLYTKGSTWKGKSTQSTEGK TS
    • Molecular weight
      30 kDa including tags
    • Amino acids
      1 to 252
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab157274 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Specific Activity: 2.35 U/mg.
  • Applications

    Functional Studies

    SDS-PAGE

  • Purity
    >95% by SDS-PAGE .

  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.02% DTT, 0.79% Tris HCl, 10% Glycerol, 1.75% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • NaMN adenylyltransferase 3
    • Nicotinamide mononucleotide adenylyltransferase 3
    • Nicotinamide nucleotide adenylyltransferase 3
    • Nicotinate-nucleotide adenylyltransferase 3
    • NMN adenylyltransferase 3
    • NMNA3_HUMAN
    • NMNAT 3
    • Nmnat3
    • PNAT 3
    • PNAT-3
    • PNAT3
    • Pyridine nucleotide adenylyltransferase 3
    see all
  • Function
    Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate with the same efficiency. Can use triazofurin monophosphate (TrMP) as substrate. Can also use GTP and ITP as nucleotide donors. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD(+). For the pyrophosphorolytic activity, can use NAD (+), NADH, NAAD, nicotinic acid adenine dinucleotide phosphate (NHD), nicotinamide guanine dinucleotide (NGD) as substrates. Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NAADP(+). Protects against axonal degeneration following injury.
  • Tissue specificity
    Expressed in lung and spleen with lower levels in placenta and kidney.
  • Pathway
    Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from nicotinamide D-ribonucleotide: step 1/1.
  • Sequence similarities
    Belongs to the eukaryotic NMN adenylyltransferase family.
  • Cellular localization
    Mitochondrion.
  • Information by UniProt

References

ab157274 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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