Recombinant Human Nogo protein (ab163499)
Key features and details
- Expression system: Wheat germ
- Tags: GST tag N-Terminus
- Suitable for: WB, ELISA
Description
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Product name
Recombinant Human Nogo protein
See all Nogo proteins and peptides -
Expression system
Wheat germ -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MEDLDQSPLVSSSDSPPRPQPAFKYQFVREPEDEEEEEEEEEEDEDEDLE ELEVLERKPAAGLSAAPVPTAPAAGAPLMDFGNDFVPPAPRGPLPAAPPV APERQPSWDPSPVSSTVPAPSPLSAAAVSPSKLPEDDEPPARPPPPPPAS VSPQAEPVWTPPAPAPAAPPSTPAAPKRRGSSGSVDETLFALPAASEPVI RSSAENMDLKEQPGNTISAGQEDFPSVLLETAASLPSLSPLSAASFKEHE YLGNLSTVLPTEGTLQENVSEASKEVSEKAKTLLIDRDLTEFSELEYSEM GSSFSVSPKAESAVIVANPREEIIVKNKDEEEKLVSNNILHNQQELPTAL TKLVKEDEVVSSEKAKDSFNEKRVAVEAPMREEYADFKPFERVWEVKDSK EDSDMLAAGGKIESNLESKVDKKCFADSLEQTNHEKDSESSNDDTSFPST PEGIKDRSGAYITCAPFNPAATESIATNIFPLLGDPTSENKTDEKKIEEK KAQIVTEKNTSTKTSNPFLVAAQDSETDYVTTDNLTKVTEEVVANMPEGL TPDLVQEACESELNEVTGTKIAYETKMDLVQTSEVMQESLYPAAQLCPSF EESEATPSPVLPDIVMEAPLNSAVPSAGASVIQPSSSPLEASSVNYESIK HEPENPPPYEEAMSVSLKKVSGIKEEIKEPENINAALQETEAPYISIACD LIKETKLSAEPAPDFSDYSEMAKVEQPVPDHSELVEDSSPDSEPVDLFSD DSIPDVPQKQDETVMLVKESLTETSFESMIEYENKEKLSALPPEGGKPYL ESFKLSLDNTKDTLLPDEVSTLSKKEKIPLQMEELSTAVYSNDDLFISKE AQIRETETFSDSSPIEIIDEFPTLISSKTDSFSKLAREYTDLEVSHKSEI ANAPDGAGSLPCTELPHDLSLKNIQPKVEEKISFSDDFSKNGSATSKVLL LPPDVSALATQAEIESIVKPKVLVKEAEKKLPSDTEKEDRSPSAIFSAEL SKTSVVDLLYWRDIKKTGVVFGASLFLLLSLTVFSIVSVTAYIALALLSV TISFRIYKGVIQAIQKSDEGHPFRAYLESEVAISEELVQKYSNSALGHVN CTIKELRRLFLVDDLVDSLKFAVLMWVFTYVGALFNGLTLLILALISLFS VPVIYERHQAQIDHYLGLANKNVKDAMAKIQAKIPGLKRKAE -
Amino acids
1 to 1192 -
Tags
GST tag N-Terminus
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Specifications
Our Abpromise guarantee covers the use of ab163499 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
ELISA
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Form
Liquid -
Additional notes
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00
Constituents: 0.31% Glutathione, 0.79% Tris HCl
General Info
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Alternative names
- 1110020G17Rik
- AA407876
- AA409940
see all -
Function
Developmental neurite growth regulatory factor with a role as a negative regulator of axon-axon adhesion and growth, and as a facilitator of neurite branching. Regulates neurite fasciculation, branching and extension in the developing nervous system. Involved in down-regulation of growth, stabilization of wiring and restriction of plasticity in the adult CNS. Regulates the radial migration of cortical neurons via an RTN4R-LINGO1 containing receptor complex (By similarity). Isoform 2 reduces the anti-apoptotic activity of Bcl-xl and Bcl-2. This is likely consecutive to their change in subcellular location, from the mitochondria to the endoplasmic reticulum, after binding and sequestration. Isoform 2 and isoform 3 inhibit BACE1 activity and amyloid precursor protein processing. -
Tissue specificity
Isoform 1 is specifically expressed in brain and testis and weakly in heart and skeletal muscle. Isoform 2 is widely expressed except for the liver. Isoform 3 is expressed in brain, skeletal muscle and adipocytes. Isoform 4 is testis-specific. -
Sequence similarities
Contains 1 reticulon domain. -
Domain
Three regions, residues 59-172, 544-725 and the loop 66 amino acids, between the two transmembrane domains, known as Nogo-66 loop, appear to be responsible for the inhibitory effect on neurite outgrowth and the spreading of neurons. This Nogo-66 loop, mediates also the binding of RTN4 to its receptor. -
Cellular localization
Endoplasmic reticulum membrane. Anchored to the membrane of the endoplasmic reticulum through 2 putative transmembrane domains. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab163499 has been referenced in 1 publication.
- Baya Mdzomba J et al. Nogo-A-targeting antibody promotes visual recovery and inhibits neuroinflammation after retinal injury. Cell Death Dis 11:101 (2020). PubMed: 32029703