Recombinant Human Notch1 protein (ab114178)



  • NatureRecombinant
  • SourceWheat germ
  • Amino Acid Sequence
    • AccessionP46531
    • SpeciesHuman
    • Molecular weight38 kDa including tags
    • Amino acids23 to 132

Associated products


Our Abpromise guarantee covers the use of ab114178 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications


    Western blot


  • FormLiquid
  • Additional notesProtein concentration is above or equal to 0.05 mg/ml.
    Best used within three months from the date of receipt.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.3% Glutathione, 0.79% Tris HCl

General Info

  • Alternative names
    • 9930111A19Rik
    • AOS5
    • AOVD1
    • hN1
    • Lin-12
    • LIN12
    • Mis6
    • Motch A
    • mT14
    • Neurogenic locus notch homolog protein 1
    • Neurogenic locus notch protein homolog
    • NICD
    • NOTCH
    • Notch 1
    • Notch 1 intracellular domain
    • Notch gene homolog 1 (Drosophila)
    • Notch homolog 1, translocation-associated (Drosophila)
    • NOTCH, Drosophila, homolog of, 1
    • notch1
    • TAN1
    • Translocation associated notch homolog
    • Translocation associated notch protein TAN 1
    • Translocation-associated notch protein TAN-1
    • xotch
    see all
  • FunctionFunctions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May be important for normal lymphocyte function. In altered form, may contribute to transformation or progression in some T-cell neoplasms. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. May be important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, may function as a receptor for neuronal DNER and may be involved in the differentiation of Bergmann glia.
  • Tissue specificityIn fetal tissues most abundant in spleen, brain stem and lung. Also present in most adult tissues where it is found mainly in lymphoid tissues.
  • Involvement in diseaseDefects in NOTCH1 are a cause of bicuspid aortic valve (BAV) [MIM:109730]. A common defect in the aortic valve in which two rather than three leaflets are present. It is often associated with aortic valve calcification and insufficiency. In extreme cases, the blood flow may be so restricted that the left ventricle fails to grow, resulting in hypoplastic left heart syndrome.
  • Sequence similaritiesBelongs to the NOTCH family.
    Contains 5 ANK repeats.
    Contains 36 EGF-like domains.
    Contains 3 LNR (Lin/Notch) repeats.
  • Post-translational
    Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane.
    O-glycosylated on the EGF-like domains. Contains both O-linked fucose and O-linked glucose.
    Ubiquitinated; undergoes 'Lys-29'-linked polyubiquitination catalyzed by ITCH.
  • Cellular localizationCell membrane and Nucleus. Following proteolytical processing NICD is translocated to the nucleus.
  • Information by UniProt

Recombinant Human Notch1 protein images

  • ab114178 on a 12.5% SDS-PAGE Stained with Coomassie Blue.

References for Recombinant Human Notch1 protein (ab114178)

ab114178 has not yet been referenced specifically in any publications.

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