Recombinant Human NSMase2 protein (ab112416)
Key features and details
- Expression system: Wheat germ
- Suitable for: SDS-PAGE, WB, ELISA
Description
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Product name
Recombinant Human NSMase2 protein -
Biological activity
useful for Antibody Production and Protein Array -
Expression system
Wheat germ -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
98 kDa including tags -
Amino acids
1 to 655
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab112416 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Western blot
ELISA
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Form
Liquid -
Additional notes
This product is useful for Antibody Production and Protein Array.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00
Constituents: 0.31% Glutathione, 0.79% Tris HCl
(Glutathione is reduced)
General Info
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Alternative names
- Cca1
- Confluent 3Y1 cell-associated protein 1
- Neutral sphingomyelinase 2
see all -
Function
Catalyzes the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Ceramide mediates numerous cellular functions, such as apoptosis and growth arrest, and is capable of regulating these 2 cellular events independently. Also hydrolyzes sphingosylphosphocholine. Regulates the cell cycle by acting as a growth suppressor in confluent cells. Probably acts as a regulator of postnatal development and participates in bone and dentin mineralization. -
Tissue specificity
Predominantly expressed in brain. -
Sequence similarities
Belongs to the neutral sphingomyelinase family. -
Developmental stage
Up-regulated during G0/G1 phases. -
Cellular localization
Golgi apparatus membrane. Cell membrane. May localize to detergent-resistant subdomains of Golgi membranes of hypothalamic neurosecretory neurons. According to PubMed:15051724, it localizes to plasma membrane in confluent contact-inhibited cells. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab112416 has been referenced in 1 publication.
- Xia J et al. Shufeng Jiedu Capsule and its active ingredients induce apoptosis, inhibit migration and invasion, and enhances doxorubicin therapeutic efficacy in hepatocellular carcinoma. Biomed Pharmacother 99:921-930 (2018). PubMed: 29710492