Recombinant Human NUDT21 protein (ab104669)

Overview

  • Product name
    Recombinant Human NUDT21 protein
  • Protein length
    Full length protein

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MGSSHHHHHHSSGLVPRGSHMSVVPPNRSQTGWPRGVTQFGNKYIQQTKP LTLERTINLYPLTNYTFGTKEPLYEKDSSVAARFQRMREEFDKIGMRRTV EGVLIVHEHRLPHVLLLQLGTTFFKLPGGELNPGEDEVEGLKRLMTEILG RQDGVLQDWVIDDCIGNWWRPNFEPPQYPYIPAHITKPKEHKKLFLVQLQ EKALFAVPKNYKLVAAPLFELYDNAPGYGPIISSLPQLLSRFNFIYN
    • Molecular weight
      28 kDa including tags
    • Amino acids
      1 to 227
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab104669 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry
    MALDI-TOF
  • Purity
    > 90 % SDS-PAGE.
    ab104669 is purified by using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 20% Glycerol, 0.2M Sodium chloride, 20mM Tris HCl, 2mM DTT, pH 8.0

General Info

  • Alternative names
    • CFIM 25
    • CFIM25
    • Cleavage and polyadenylation specific factor 5
    • Cleavage and polyadenylation specific factor 5 25 kD subunit
    • Cleavage and polyadenylation specific factor 5 25 kDa
    • Cleavage and polyadenylation specificity factor 25 kDa subunit
    • Cleavage and polyadenylation specificity factor subunit 5
    • Cleavage factor Im complex 25 kDa subunit
    • CPSF 25
    • CPSF 25 kDa subunit
    • CPSF 5
    • CPSF25
    • CPSF5
    • CPSF5_HUMAN
    • DKFZp686H1588
    • Nucleoside diphosphate linked moiety X motif 21
    • Nucleoside diphosphate-linked moiety X motif 21
    • Nudix (nucleoside diphosphate linked moiety X) type motif 21
    • Nudix hydrolase 21
    • Nudix motif 21
    • NUDT 21
    • Nudt21
    • Pre mRNA cleavage factor Im (25kD)
    • Pre mRNA cleavage factor Im 25 kDa subunit
    • Pre mRNA cleavage factor Im 25kD subunit
    • Pre-mRNA cleavage factor Im 25 kDa subunit
    see all
  • Function
    Component of the cleavage factor Im (CFIm) complex that plays a key role in pre-mRNA 3'-processing. Involved in association with CPSF6 or CPSF7 in pre-MRNA 3'-end poly(A) site cleavage and poly(A) addition. NUDT21/CPSF5 binds to cleavage and polyadenylation RNA substrates. The homodimer mediates simultaneous sequence-specific recognition of two 5'-UGUA-3' elements within the pre-mRNA. Binds to, but does not hydrolyze mono- and di-adenosine nucleotides. May have a role in mRNA export.
  • Sequence similarities
    Belongs to the Nudix hydrolase family. CPSF5 subfamily.
    Contains 1 nudix hydrolase domain.
  • Post-translational
    modifications
    Acetylated mainly by p300/CBP, recruited to the complex by CPSF6. Acetylation decreases interaction with PAPAO. Deacetylated by the class I/II HDACs, HDAC1, HDAC3 and HDAC10, and by the class III HDACs, SIRT1 AND SIRT2.
  • Cellular localization
    Nucleus. In punctate subnuclear structures localized adjacent to nuclear speckles, called paraspeckles.
  • Information by UniProt

Images

  • 15% SDS-PAGE showing ab104669 at approximately 28.3kDa (3µg).

References

ab104669 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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