Recombinant Human Oct4 protein (ab95845)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionQ01860
    • SpeciesHuman
    • Molecular weight23 kDa
    • Amino acids1 to 212

Specifications

Our Abpromise guarantee covers the use of ab95845 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    Dot blot

    SDS-PAGE

    Indirect ELISA

  • Purity> 90 % SDS-PAGE.
    ab95845 was refolded to soluble form.
  • FormLiquid
  • Additional notes

    This product is for Research Use Only and not to be used for any commercial resale purpose, including, but not limited to, using the product as part of any developed assay technology such as ELISA, ELISPOT, or Multiplex assay that is sold to another end-user.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 5% Glycerol, PBS (1.5% Monobasic dihydrogen potassium phosphate, 8.1mM Dibasic monohydrogen sodium phosphate, 500mM Sodium chloride, 2.7mM Potassium chloride, pH 7.4)

General Info

  • Alternative names
    • Octamer binding transcription factor 4
    • MGC22487
    • Oct 3
    • Oct 4
    • Oct-3
    • Oct-4
    • OCT3
    • Oct4
    • Octamer binding protein 3
    • Octamer binding protein 4
    • Octamer binding transcription factor 3
    • Octamer-binding protein 3
    • Octamer-binding protein 4
    • Octamer-binding transcription factor 3
    • OTF 3
    • OTF 4
    • OTF-3
    • OTF3
    • OTF4
    • PO5F1_HUMAN
    • POU class 5 homeobox 1
    • POU domain class 5 transcription factor 1
    • POU domain transcription factor OCT4
    • POU domain, class 5, transcription factor 1
    • POU-type homeodomain-containing DNA-binding protein
    • POU5F1
    see all
  • FunctionTranscription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
  • Tissue specificityExpressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
  • Sequence similaritiesBelongs to the POU transcription factor family. Class-5 subfamily.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 POU-specific domain.
  • Developmental stageHighly expressed in undifferentiated embryonic stem cells and expression decreases gradually after embryoid body (EB) formation.
  • DomainThe POU-specific domain mediates interaction with PKM2.
  • Post-translational
    modifications
    Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression.
    Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation.
  • Cellular localizationNucleus. Expressed in a diffuse and slightly punctuate pattern.
  • Information by UniProt

Recombinant Human Oct4 protein images

  • SDS-PAGE analysis of 5µg ab95845
  • Indirect ELISA detection of immobilized ab95845.

References for Recombinant Human Oct4 protein (ab95845)

ab95845 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting Abcam.

I have identified a pair of antibodies that have been tested and guaranteed to work in sandwich ELISA against the human Oct4 protein, a protein involved in stem cell self-renewal. Links to the antibodies are giv...

Read More

This Oct3/4 is Isoform 1; the cDNA clone used to produce it is Transcript Variant 1- mRNA Reference Sequence NM_002701.3, http://www.ncbi.nlm.nih.gov/nuccore/54112126

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"