Recombinant Human Ornithine Carbamoyltransferase protein (ab123452)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionP00480
    • SpeciesHuman
    • SequenceMLFNLRILLN NAAFRNGHNF MVRNFRCGQP LQNKVQLKGR DLLTLKNFTG EEIKYMLWLS ADLKFRIKQK GEYLPLLQGK SLGMIFEKRS TRTRLSTETG FALLGGHPCF LTTQDIHLGV NESLTDTARV LSSMADAVLA RVYKQSDLDT LAKEASIPII NGLSDLYHPI QILADYLTLQ EHYSSLKGLT LSWIGDGNNI LHSIMMSAAK FGMHLQAATP KGYEPDASVT KLAEQYAKEN GTKLLLTNDP LEAAHGGNVL ITDTWISMGQ EEEKKKRLQA FQGYQVTMKT AKVAASDWTF LHCLPRKPEE VDDEVFYSPR SLVFPEAENR KWTIMAVMVS LLTDYSPQLQKPKF
    • Molecular weight40 kDa
    • Amino acids1 to 354
    • TagsHis tag C-Terminus

Specifications

Our Abpromise guarantee covers the use of ab123452 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Western blot

    ELISA

  • Purity> 80 % SDS-PAGE.

  • FormLiquid
  • Additional notes

    Product was previously marketed under the MitoSciences sub-brand.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Constituents: 0.79% Tris HCl, 0.15% EDTA, 10% Glycerol, 0.88% Sodium chloride

General Info

  • Alternative names
    • EC 2.1.3.3
    • MGC129967
    • MGC129968
    • MGC138856
    • OCTD
    • Ornithine carbamoyltransferase mitochondrial
    • Ornithine carbamoyltransferase, mitochondrial
    • Ornithine transcarbamylase
    • Otc
    • OTC_HUMAN
    • OTCase
    see all
  • Tissue specificityMainly expressed in liver and intestinal mucosa.
  • PathwayNitrogen metabolism; urea cycle; L-citrulline from L-ornithine and carbamoyl phosphate: step 1/1.
  • Involvement in diseaseOrnithine carbamoyltransferase deficiency
  • Sequence similaritiesBelongs to the ATCase/OTCase family.
  • Post-translational
    modifications
    Acetylation at Lys-88 negatively regulates ornithine carbamoyltransferase activity in response to nutrient signals.
  • Cellular localizationMitochondrion matrix.
  • Information by UniProt

Recombinant Human Ornithine Carbamoyltransferase protein images

  • 10-20% acrylamide separation gel showing ab123452 at approximately 40kDa (1µg).
  • Anti-Ornithine Carbamoyltransferase antibody (ab86665) at 0.5 µg/ml + Recombinant Human Ornithine Carbamoyltransferase protein (ab123452) at 0.01 µg

    Secondary
    Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/10000 dilution
    Developed using the ECL technique

    Predicted band size : 40 kDa
    Observed band size : 40 kDa


    Exposure time : 5 minutes

References for Recombinant Human Ornithine Carbamoyltransferase protein (ab123452)

ab123452 has not yet been referenced specifically in any publications.

Product Wall

Ab58953:
Amino acids 26-530 of UniProt Q5DT02
GKLLV VPMDGSHWFT MQSVVEKLIL RGHEVVVVMP EVSWQLERSL NCTVKTYSTS YTLEDQNREF MVFAHAQWKA QAQSIFSLLM SSSSGFLDLF FSHCRSLFND RKLVEYLKES SFDAVFLDPF DTCGLIVAKY FSLPSVVFTR GIFCHHLEEG AQCPAPLSYV PNDLLGFSDA MTF...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"