Recombinant Human p35 + p25 protein (ab125653)

Overview

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • AccessionQ15078
    • SpeciesHuman
    • Molecular weight49 kDa including tags
    • Amino acids98 to 307

Specifications

Our Abpromise guarantee covers the use of ab125653 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

  • Purity> 85 % Densitometry.
    Purity was determined to be >85% by densitometry.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride

General Info

  • Alternative names
    • CDK5
    • CDK5 activator 1
    • CDK5P35
    • CDK5R
    • CDK5R1
    • Cyclin dependent kinase 5 activator 1
    • Cyclin dependent kinase 5 regulatory subunit 1
    • Cyclin dependent kinase 5, regulatory subunit 1 (p35)
    • Cyclin-dependent kinase 5
    • NCK5A
    • Neuronal CDK5 activator
    • p23
    • p25
    • p35
    • P35nck5a
    • PSSALRE
    • Regulatory partner for CDK5 kinase
    • Tau protein kinase II 23kDa subunit
    • TPKII regulatory subunit
    see all
  • Relevancep35 is a neuron-specific activator of cyclin-dependent kinase 5 (CDK5); the activation of CDK5 is required for proper development of the central nervous system. The p35 form of this protein is proteolytically cleaved by calpain, generating a p25 form. P25 deregulates CDK5 activity by prolonging its activation and changing its cellular location. The p25 form accumulates in the brain neurons of patients with Alzheimer's disease. This accumulation correlates with an increase in CDK5 kinase activity, and may lead to aberrantly phosphorylated forms of the microtubule-associated protein tau, which contributes to Alzheimer's disease.
  • Cellular localizationCell Membrane, Cytoplasmic and Nuclear

Recombinant Human p35 + p25 protein images

  • SDS-PAGE analysis of ab125653.

References for Recombinant Human p35 + p25 protein (ab125653)

ab125653 has not yet been referenced specifically in any publications.

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