Recombinant Human p35 + p25 protein (ab60761)



  • Nature
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Species

Associated products


Our Abpromise guarantee covers the use of ab60761 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    Functional Studies


  • Purity
    > 90 % SDS-PAGE.
    Purity: >90% as determined by densitometry.
  • Form
  • Additional notes

    ab89813 (Histone H1 protein) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

General Info

  • Alternative names
    • CDK5
    • CDK5 activator 1
    • CDK5P35
    • CDK5R
    • CDK5R1
    • Cyclin dependent kinase 5 activator 1
    • Cyclin dependent kinase 5 regulatory subunit 1
    • Cyclin dependent kinase 5, regulatory subunit 1 (p35)
    • Cyclin-dependent kinase 5
    • NCK5A
    • Neuronal CDK5 activator
    • p23
    • p25
    • p35
    • P35nck5a
    • Regulatory partner for CDK5 kinase
    • Tau protein kinase II 23kDa subunit
    • TPKII regulatory subunit
    see all
  • Relevance
    p35 is a neuron-specific activator of cyclin-dependent kinase 5 (CDK5); the activation of CDK5 is required for proper development of the central nervous system. The p35 form of this protein is proteolytically cleaved by calpain, generating a p25 form. P25 deregulates CDK5 activity by prolonging its activation and changing its cellular location. The p25 form accumulates in the brain neurons of patients with Alzheimer's disease. This accumulation correlates with an increase in CDK5 kinase activity, and may lead to aberrantly phosphorylated forms of the microtubule-associated protein tau, which contributes to Alzheimer's disease.
  • Cellular localization
    Cell Membrane, Cytoplasmic and Nuclear


  • Sample Kinase Activity Plot.
  • ab60761 on SDS-PAGE, p35 MW 59kDa and p25 49kDa.


ab60761 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Western blot
Review text: The blot image provided in this review has mouse Dorsal Striatum loaded at 70ug in lanes 2-7 and the P35/P25-GST lysate in .5, 1 and 3 ug in lanes 8-10. The .5ug amount appears to be the least necessary to produce both the p35-GST and p25-GST bands. The p25-GST band is much stronger than the p35-GST band. A goat polyclonal anti-GST antibody was used from GE Health sciences. Therefore, lanes 8-10 have a yellow band because of co-labeling of p35/p25 and the GST tag. There was some nonspecific binding with the p35/p25-GST lysate due to such a high amount being used. These bands are found mainly below the p25 band. There was also nonspecific binding with the anti-GST antibody within the p35/p25-GST lysate but not the Dorsal Striatum lysates. Only p35 appears in the Dorsal Striatum lanes because the 10% gel was not able to resolve p25 because it is too light. 12.5% gels have been used to resolve both p35 and p25 in previous studies, but this percent gel would not have been able to resolve the GST tagged proteins. A gradient gel would be necessary.
Sample: Mouse Cell lysate - whole cell (Brain)
Loading amount: 70 µg
Specification: Brain
Gel Running Conditions: Reduced Denaturing (10% gel)
Blocking step: Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details
Incubation time: 18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk

Primary antibody (in addition to 'p35 + p25 protein (Tagged)')
Name: Non-Abcam antibody was used: CST anti-p35/p25
Host species: Rabbit
Clonality: Monyclonal
Conjugation: Other
Dilution: 1/1000

Secondary antibody
Name: Non-Abcam antibody was used: Licor 700
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 700DX

Detection method: Licor
Exposure: 1 hour(s), 0 minute(s) and 0 second(s)
Bands: Specific: 59 and 49 kDa Non-specific: several below 40 kDa
Positive control: The p35/p25-GST lysate served as a positive control
Negative control: None

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Verified customer

Submitted Apr 08 2011


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