Overview

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • AccessionQ15078
    • SpeciesHuman
    • Molecular weight60 kDa including tags
    • Amino acids1 to 307

Specifications

Our Abpromise guarantee covers the use of ab119724 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

  • Purity> 70 % SDS-PAGE.
    The purity was determined to be 70% by densitometry
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 25% Glycerol, 0.29% Sodium chloride, 0.79% Tris HCl, 0.003% EDTA, 0.003% DTT, 0.31% Glutathione, 0.002% PMSF

General Info

  • Alternative names
    • CD5R1_HUMAN
    • CDK5 activator 1
    • CDK5P35
    • CDK5R
    • CDK5R1
    • Cyclin dependent kinase 5 regulatory subunit 1
    • Cyclin-dependent kinase 5 activator 1
    • Cyclin-dependent kinase 5 regulatory subunit 1
    • NCK 5A
    • Neuronal CDK5 activator
    • p23
    • p25
    • p35
    • Regulatory partner for CDK5 kinase
    • Tau protein kinase II 23 kDa subunit
    • TPKII regulatory subunit
    see all
  • Functionp35 is a neuron specific activator of CDK5. The complex p35/CDK5 is required for neurite outgrowth and cortical lamination. Activator of TPKII.
  • Tissue specificityBrain and neuron specific.
  • Sequence similaritiesBelongs to the cyclin-dependent kinase 5 activator family.
  • Post-translational
    modifications
    The p35 form is proteolytically cleaved by calpain, giving rise to the p25 form. P35 has a 5 to 10 fold shorter half-life compared to p25. The conversion results in deregulation of the CDK5 kinase: p25/CDK5 kinase displays an increased and altered tau phosphorylation in comparison to the p35/CDK5 kinase in vivo.
    Probably myristoylated. The Gly-2-Ala mutant is absent of the cell periphery, suggesting that a proper myristoylation signal is essential for the proper distribution of p35.
  • Cellular localizationCell membrane. In the primary cortical neurons, p35 is present in the peripheries and nerve terminals and Nucleus. Cytoplasm > perinuclear region. The conversion of p35 to p25 relocalizes the protein from the cell periphery to the cytoplasm, in nuclear and perinuclear regions. In the primary cortical neurons, p25 is primarily concentrated in the cell soma and is largely absent from neurites.
  • Information by UniProt

Recombinant Human p35 protein images

  • SDS Page analysis of ab119724.

References for Recombinant Human p35 protein (ab119724)

ab119724 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab119724.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"