Recombinant Human p38 protein (ab45158)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Human

Specifications

Our Abpromise guarantee covers the use of ab45158 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Purity
    > 90 % SDS-PAGE.

  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

General Info

  • Alternative names
    • CSAID binding protein
    • CSAID Binding Protein 1
    • CSAID-binding protein
    • Csaids binding protein
    • CSBP
    • CSBP 1
    • CSBP 2
    • CSBP1
    • CSBP2
    • CSPB1
    • Cytokine suppressive anti-inflammatory drug-binding protein
    • EXIP
    • MAP kinase 14
    • MAP kinase MXI2
    • MAP kinase p38 alpha
    • MAPK 14
    • MAPK14
    • MAX interacting protein 2
    • MAX-interacting protein 2
    • Mitogen Activated Protein Kinase 14
    • Mitogen activated protein kinase p38 alpha
    • Mitogen-activated protein kinase 14
    • Mitogen-activated protein kinase p38 alpha
    • MK14_HUMAN
    • Mxi 2
    • MXI2
    • p38
    • p38 ALPHA
    • p38 MAP kinase
    • p38 MAPK
    • p38 mitogen activated protein kinase
    • p38ALPHA
    • p38alpha Exip
    • PRKM14
    • PRKM15
    • RK
    • SAPK2A
    see all
  • Function
    Responds to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis.
  • Tissue specificity
    Brain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • Domain
    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications
    Dually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt

References for Recombinant Human p38 protein (ab45158)

ab45158 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting Abcam. Based on the bacterial expression condition, this protein is not expected to have high phosphorylation level. Also, the size that is reported is based on the size seen when the protein was run on a reduced SDS-PAGE gel w...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up