Recombinant human p38 protein (ab82188)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesHuman
    • Molecular weight43 kDa including tags
    • Amino acids1 to 360
    • TagsHis tag C-Terminus

Specifications

Our Abpromise guarantee covers the use of ab82188 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • FormLiquid
  • Additional notesab82188 contains no detectable proteases, DNase and RNase activity.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 20% Glycerol, 20mM Tris Cl, 100mM Potassium chloride, 1mM DTT, 0.2mM EDTA, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • CSAID binding protein
    • CSAID Binding Protein 1
    • CSAID-binding protein
    • Csaids binding protein
    • CSBP
    • CSBP 1
    • CSBP 2
    • CSBP1
    • CSBP2
    • CSPB1
    • Cytokine suppressive anti-inflammatory drug-binding protein
    • EXIP
    • MAP kinase 14
    • MAP kinase MXI2
    • MAP kinase p38 alpha
    • MAPK 14
    • MAPK14
    • MAX interacting protein 2
    • MAX-interacting protein 2
    • Mitogen Activated Protein Kinase 14
    • Mitogen activated protein kinase p38 alpha
    • Mitogen-activated protein kinase 14
    • Mitogen-activated protein kinase p38 alpha
    • MK14_HUMAN
    • Mxi 2
    • MXI2
    • p38
    • p38 ALPHA
    • p38 MAP kinase
    • p38 MAPK
    • p38 mitogen activated protein kinase
    • p38ALPHA
    • p38alpha Exip
    • PRKM14
    • PRKM15
    • RK
    • SAPK2A
    see all
  • FunctionResponds to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis.
  • Tissue specificityBrain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications
    Dually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt

References for Recombinant human p38 protein (ab82188)

ab82188 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting Abcam. I have heard from the alb about the p38 protein, ab82118. They have said it should be fine to remove the TRIS and DTT from the buffer by dialyzing against PBS. Please do the dialysis at 4C and use the protein immediately...

Read More

It is not known if this protein is phosphorylated or not. The recombinant protein was expressed in E. coli cells and purified using IMAC affinity column followed by SEC FPLC in Tris-HCl and KCl buffer containing 10% glycerol.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"