The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activityN terminal His tagged protein.
% SDS-PAGE. Purified by an affinity column in combination with FPLC chromatography.
The purified recombinant protein is greater than 95% homogeneous and contains no detectable protease, DNase and RNase activity.
Additional notesN terminal His tagged protein.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Cytokine suppressive anti-inflammatory drug-binding protein
MAP kinase 14
MAP kinase MXI2
MAP kinase p38 alpha
MAX interacting protein 2
MAX-interacting protein 2
Mitogen Activated Protein Kinase 14
Mitogen activated protein kinase p38 alpha
Mitogen-activated protein kinase 14
Mitogen-activated protein kinase p38 alpha
p38 MAP kinase
p38 mitogen activated protein kinase
FunctionResponds to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis.
Tissue specificityBrain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney.
Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. Contains 1 protein kinase domain.
DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Post-translational modificationsDually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme. Phosphorylated upon DNA damage, probably by ATM or ATR.