The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Protein concentration is above or equal to 0.05 mg/ml.
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Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00 Constituents: 0.31% Glutathione, 0.79% Tris HCl
CAF 1 subunit B
CAF I 60 kDa subunit
CAF-1 subunit B
CAF-I 60 kDa subunit
Chromatin assembly factor 1 subunit B
Chromatin assembly factor I
Chromatin assembly factor I p60 subunit
Human chromatin assembly factor-I p60 subunit
M phase phosphoprotein 7
M-phase phosphoprotein 7
Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. The CCR4-NOT complex functions as general transcription regulation complex.
Belongs to the WD repeat HIR1 family. Contains 7 WD repeats.
Active complex is found in G1, S and G2 phases.
Differentially phosphorylated during cell cycle. During mitosis the p60 subunit of inactive CAF-1 is hyperphosphorylated and displaced into the cytosol. Progressivly dephosphorylated from G1 to S and G2 phase. Phosphorylated p60 is recruited to chromatin undergoing DNA repair after UV irradiation in G1, S or G2 phases.
Nucleus. Cytoplasm. DNA replication foci. Cytoplasmic in M phase.