Recombinant Human p73 beta protein (ab102553)

Overview

  • Product nameRecombinant Human p73 beta protein
  • Protein lengthFull length protein

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • AccessionO15350-2
    • SpeciesHuman
    • Molecular weight88 kDa including tags
    • Amino acids1 to 499

Specifications

Our Abpromise guarantee covers the use of ab102553 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Western blot

  • Purity> 90 % SDS-PAGE.
    Purity was determined to be >90% by densitometry.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

General Info

  • Alternative names
    • p53 related protein
    • P73
    • TP73
    • Tumor protein p73
  • RelevanceThe p73 exhibits sequence homology to the p53's transcriptional activation, DNA binding, and oligomerization domains. However, adenovirus E1B 55K, simian virus 40 T, and human papillomavirus E6 do not physically interact with p73 indicating that the functions of these two proteins may differ under physiological conditions. Significant homology between p53, p63, and p73 (approximately 63% amino acid identity in the DNA binding domain suggest that they may have overlapping functions in the regulation of gene expression. p73 is expressed in either a full length form (a) (MW ~80kD) or a shorter (b) (MW ~70kD) mRNA variant, with exon 13 spliced out. Transient overexpression of p73 in cells can induce apoptosis and p21, a cellular p53 target gene primarily responsible for p53 dependent cell cycle arrest. The targeted disruption of p73 gene leads to defects hippocampal dysgenesis, hydrocephalus, chronic inflammation and infections. p73 is upregulated in a significant fraction of anaplastic thyroid cancers, whereas it is not detectable in normal thyroid epithelial cells nor in papillary or follicular thyroid cancer.
  • Cellular localizationNuclear

Recombinant Human p73 beta protein images

  • SDS-PAGE showing ab102553 at approximately 88kDa.

References for Recombinant Human p73 beta protein (ab102553)

ab102553 has not yet been referenced specifically in any publications.

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