Recombinant Human p75 NGF Receptor protein (Fc Chimera) (ab83679)

Overview

Description

  • NatureRecombinant
  • SourceHEK 293 cells
  • Amino Acid Sequence
    • AccessionP08138
    • SpeciesHuman
    • SequenceKEACPTGLYTHSGECCKACNLGEGVAQPCGANQTVCEPCLDSVTFSDVVS ATEPCKPC TECVGLQSMSAPCVEADDAVCRCAYGYYQDETTGRCEACR VCEAGSGLVFSCQDKQN TVCEECPDGTYSDEANHVDPCLPCTVCEDTE RQLRECTRWADAECEEIPGRWITRSTP PEGSDSTAPSTQEPEAPPEQD LIASTVAGVVTTVMGIPKVDKKVEPKSCDKTHTCPPCP APELLGGPSV FLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKT KPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCRVSNKALPAPIEKTISK AKGQPREPQV YTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQ PENNYKTTPPVLDSDGSFFLY SKLTVDKSRWQQGNVFSCSVMHEALHN HYTQKSLSLSPGK
    • Amino acids1 to 237
    • Additional sequence informationFused with the Fc region of Human IgG1 at the C-terminus.

Specifications

Our Abpromise guarantee covers the use of ab83679 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Purity> 95 % SDS-PAGE.

  • FormLyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Trehalose, 1% Human serum albumin, PBS

  • ReconstitutionIt is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.

General Info

  • Alternative names
    • CD271
    • CD271 antigen
    • Gp80 LNGFR
    • Gp80-LNGFR
    • Low affinity nerve growth factor receptor
    • Low affinity neurotrophin receptor p75NTR
    • Low-affinity nerve growth factor receptor
    • Nerve growth factor receptor
    • Nerve growth factor receptor TNFR superfamily member 16
    • NGF receptor
    • Ngfr
    • p75 ICD
    • p75 Neurotrophin receptor
    • p75 NTR
    • p75(NTR)
    • p75NTR
    • TNFR Superfamily Member 16
    • TNFRSF16
    • TNR16_HUMAN
    • Tumor necrosis factor receptor superfamily member 16
    see all
  • FunctionLow affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells.
  • Sequence similaritiesContains 1 death domain.
    Contains 4 TNFR-Cys repeats.
  • DomainDeath domain is responsible for interaction with RANBP9.
    The extracellular domain is responsible for interaction with NTRK1.
  • Post-translational
    modifications
    N- and O-glycosylated.
    O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc.
    Phosphorylated on serine residues.
  • Cellular localizationMembrane.
  • Information by UniProt

Recombinant Human p75 NGF Receptor protein (Fc Chimera) images

  • Densitometry of protein isoforms visualised by 2-D. The triangle indicates the theoretical MW and pI of the protein.
  • 1D SDS-PAGE of ab83679 before and after treatment with glycosidases to remove oligosaccharides.
    Lane 1: MW markers; Lane 2: ab83679; Lane 3: ab83679 treated with PNGase F to remove potential N-linked glycans; Lane 4: ab83679 treated with a glycosidase cocktail to remove potential N and O-linked glycans. 10 μg protein loaded per lane; Deep Purple™ stained.

    Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. Subsequent drop in MW after treatment with glycosidase cocktail indicates the presence of Olinked glycans. Faint bands in lane 3 and lane 4 are glycosidase enzymes.

  • A sample of ab83679 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel. 40 μg protein loaded per lane; Deep Purple™ stained.

References for Recombinant Human p75 NGF Receptor protein (Fc Chimera) (ab83679)

ab83679 has not yet been referenced specifically in any publications.

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Currently, only the p75 NGF Receptor protein ab83678 has been shown to have biological activity. This has been performed by measuring t...

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