Recombinant human PADI1 + PADI2 +PADI3 + PADI4 + PADI6 protein (ab170409)

Overview

  • Product name
    Recombinant human PADI1 + PADI2 +PADI3 + PADI4 + PADI6 protein
  • Protein length
    Full length protein

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Species
      Human
    • Tags
      proprietary tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab170409 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Sample Mass Spectrometry Data

    Peptide Sequence PAD treated Negative control
    XXRXXXXXXXXX 5 reads, 3 citrullination No citrullination
    XXXXXXXXXXRXXXRXXXX 8 reads, 4 citrullination No citrullination
    XXXXXXRXXXXXXXX 16 reads, 6 citrullination No citrullination

  • Applications

    Functional Studies

    SDS-PAGE

  • Purity
    > 95 % Densitometry.

  • Form
    Liquid
  • Additional notes
    ab170409 is ideally suited for in vitro citrullination of protein targets. Sample Mass Spectrometry Data

    Peptide Sequence PAD treated Negative control
    XXRXXXXXXXXX 5 reads, 3 citrullination No citrullination
    XXXXXXXXXXRXXXRXXXX 8 reads, 4 citrullination No citrullination
    XXXXXXRXXXXXXXX 16 reads, 6 citrullination No citrullination

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

Images

  • SDS-PAGE analysis of ab170409.

    Lane 1: PADI1
    Lane 2: PADI4
    Lane 3: PADI3
    Lane 4: PADI6
    Lane 5: PADI2
    Lane 6: ab170409

    PADI1: approx. 95 kDa
    PADI4: approx. 96 kDa
    PADI3: approx. 97 kDa
    PADI6: approx. 100 kDa
    PADI2: approx. 99 kDa

  • Activity data using ab170409.

References

ab170409 has not yet been referenced specifically in any publications.

Customer reviews and Q&As



I can confirm that this product has been tested to citrullinate Arginine in different peptide sequences. This has been measured by Mass Spec and confirmed activity of the product (please see image on datasheet). Unfortunately, we do not have ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up