The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological Activity :
Measured by its ability to cleave a PAF analog in a chromogenic substrate linked assay. At a PAF2 concentration of 10.0 µg/ml, 50% cleavage was achieved at an incubation time of approximately 2 minutes.
< 0.100 Eu/µg
% SDS-PAGE. Purity is greater than 95% by SDS-PAGE gel and HPLC analyses.
Endotoxin level is less than 0.1 ng per µg (1EU/µg).
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Phospholipase A2, group VII (platelet activating factor acetylhydrolase, plasma)
Platelet activating factor acetylhydrolase
Platelet activating factor acetylhydrolase 2
Platelet-activating factor acetylhydrolase
Modulates the action of platelet-activating factor (PAF) by hydrolyzing the sn-2 ester bond to yield the biologically inactive lyso-PAF. Has a specificity for substrates with a short residue at the sn-2 position. It is inactive against long-chain phospholipids.