ReconstitutionReconstitute with 100 ul sterile deionized water. For long term storage or for preparation of more diluted working stock solution, a carrier protein (e.g. 1% serum albumin) should be added to the vial.
Csk binding protein
Phosphoprotein associated with glycosphingolipid enriched microdomains
Phosphoprotein associated with glycosphingolipid enriched microdomains 1
Phosphoprotein associated with glycosphingolipid microdomains 1
Phosphoprotein associated with glycosphingolipid-enriched microdomains 1
Protein Associated with Glycosphingolipid Enriched Microdomains
Transmembrane adapter protein PAG
Transmembrane phosphoprotein Cbp
FunctionNegatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Promotes CSK activation and recruitment to lipid rafts, which results in LCK inhibition. Inhibits immunological synapse formation by preventing dynamic arrangement of lipid raft proteins. May be involved in cell adhesion signaling.
Tissue specificityUbiquitously expressed. Present in germinal center B-cells, plasma cells, T-cells, monocytes and platelets (at protein level).
Post-translational modificationsPalmitoylated. Phosphorylated by FYN on Tyr-317 in resting T-cells; which promotes interaction with CSK. Dephosphorylated by PTPRC/CD45 upon TCR activation; which leads to CSK dissociation. May also be dephosphorylated by PTPN11. Hyperphosphorylated in mast cells upon FCER1 activation.
Cellular localizationCell membrane. Present in lipid rafts.
SDS PAGE analysis of ab56521: 12% separating gel, 6% stacking gel; reducing conditions. Staining with Coomasie Blue.
Lane 1-5: PAG recombinant protein (2 µg, 1 µg, 0.5 µg, 0.2 µg, 0.1 µg per lane).
Western blot - PAG protein (His tag) (ab56521)
Western Blot analysis of ab56521. Reducing conditions.
Lane 1-4: immunostaining with specific anti PAG antibody. Detection with Goat anti-rabbit / HRP secondary antibody. Amount of PAG recombinant protein loaded on each lane: (1) 0.1 µg, (2) 0.050 µg, (3) 0.020 µg, (4) 0.010 µg.
References for Recombinant Human PAG protein (ab56521)
This product has been referenced in:
Le Page A et al. Downregulation of inhibitory SRC homology 2 domain-containing phosphatase-1 (SHP-1) leads to recovery of T cell responses in elderly. Cell Commun Signal12:2 (2014).
Read more (PubMed: 24405902) »