The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
% SDS-PAGE. Human PAI1 stable mutant is reacted with an excess of Human sc-PLAT at physiological pH. The resulting 1:1 molar complex is purified from the remaining PLAT by cobalt metal affinity chromatography resin.
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Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
PAI1 acts as "bait" for tissue plasminogen activator (TPA), urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis.
TPA: This converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events.
Secreted protein; extracellular space.
SDS-PAGE - PAI1 + PLAT protein (Human) (ab92549)
Lane 1: ab92549 (3µg) Reduced
Lane 2: ab92549 (3µg) Non-Reduced
This product has been referenced in:
Liu J et al. Expression of CCR6 in esophageal squamous cell carcinoma and its effects on epithelial-to-mesenchymal transition. Oncotarget8:115244-115253 (2017).
Read more (PubMed: 29383156) »