Overview

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • SpeciesHuman

Specifications

Our Abpromise guarantee covers the use of ab67946 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    Western blot

  • Purity> 90 % SDS-PAGE.

  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Constituents: 25% Glycerol, 50mM Sodium chloride, 50mM Tris HCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

General Info

  • Alternative names
    • ADRB2
    • Alpha PAK
    • Alpha-PAK
    • MGC130000
    • MGC130001
    • p21 activated kinase 1
    • p21 protein (Cdc42/Rac) activated kinase 1
    • p21-activated kinase 1
    • p21/Cdc42/Rac1 activated kinase 1 (yeast Ste20 related)
    • p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast)
    • p65 PAK
    • p65-PAK
    • P68-PAK
    • PAK alpha
    • PAK-1
    • Pak1
    • PAK1_HUMAN
    • Paka
    • PAKalpha
    • Protein kinase MUK2
    • Rac/p21-activated kinase
    • Serine/threonine-protein kinase PAK 1
    • STE20 homolog yeast
    see all
  • FunctionThe activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.
  • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 CRIB domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated when activated by CDC42/p21 and RAC1.
  • Cellular localizationCytoplasm. Cell junction > focal adhesion. Recruited to focal adhesions upon activation.
  • Information by UniProt

Recombinant Human PAK1 protein images

  • Anti-PAK1 antibody (ab1178) at 1/1000 dilution + Recombinant Human PAK1 protein (ab67946) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds

References for Recombinant Human PAK1 protein (ab67946)

ab67946 has not yet been referenced specifically in any publications.

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