Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionQ13177
    • SpeciesHuman
    • Molecular weight33 kDa
    • Amino acids227 to 524

Specifications

Our Abpromise guarantee covers the use of ab95310 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity86 nmoles/min/µg Analysis of enzymatic activity: 1. Different concentrations of PAK2 were incubated in a buffer containing 50 mM HEPES (pH7.5), 10 mM MgCl2, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35, and 2 µM substrate (SER/THR 20) at room temperature for 1 hour. 2. Developer solution was added to reaction and reaction was stopped after 1h of incubation at RT. 3. Fluorescence was detected using ?exc=460±40 nm and ?em=528±20 nm filters.
  • Applications

    Functional Studies

  • Purity> 90 % SDS-PAGE.

  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 10% Glycerol, 5mM DTT, 25mM Tris HCl, 150mM Sodium chloride, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • C-t-PAK2
    • CB422
    • EC 2.7.11.1
    • Gamma PAK
    • Gamma-PAK
    • hPAK65
    • Kinase
    • p21 (CDKN1A) activated kinase 2
    • p21 (CDKN1A)-activated kinase 2a
    • p21 activated kinase 2
    • p21 protein (Cdc42/Rac)-activated kinase 2
    • p21 protein Cdc42 Rac activated kinase 2
    • p21-activated kinase 2
    • p21-activated kinase, 65-KD
    • p21-activated protein kinase I
    • p21CDKN1A activated kinase 2
    • p27
    • p34
    • p58
    • p65PAK
    • PAK 2
    • PAK-2
    • PAK-2p34
    • Pak2
    • PAK2_HUMAN
    • PAK65
    • PAKgamma
    • S6 H4 kinase
    • S6/H4 kinase
    • Serine threonine protein kinase PAK 2
    • Serine/threonine protein kinase PAK 2
    see all
  • FunctionThe activated kinase acts on a variety of targets. Phosphorylates ribosomal protein S6, histone H4 and myelin basic protein. Full length PAK 2 stimulates cell survival and cell growth. The process is, at least in part, mediated by phosphorylation and inhibition of pro-apoptotic BAD. Caspase-activated PAK-2p34 is involved in cell death response, probably involving the JNK signaling pathway. Cleaved PAK-2p34 seems to have a higher activity than the CDC42-activated form.
  • Tissue specificityUbiquitously expressed. Higher levels seen in skeletal muscle, ovary, thymus and spleen.
  • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 CRIB domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Full length PAK 2 is autophosphorylated when activated by CDC42/p21. Following cleavage, both peptides, PAK-2p27 and PAK-2p34, become highly autophosphorylated, with PAK-2p27 being phosphorylated on serine and PAK-2p34 on threonine residues, respectively. Autophosphorylation of PAK-2p27 can occur in the absence of any effectors and is dependent on phosphorylation of Thr-402, because PAK-2p27 is acting as an exogenous substrate.
    During apoptosis proteolytically cleaved by caspase-3 or caspase-3-like proteases to yield active PAK-2p34.
    Ubiquitinated, leading to its proteasomal degradation.
    PAK-2p34 is myristoylated.
  • Cellular localizationCytoplasm and Nucleus. Cytoplasm > perinuclear region. Membrane. Interaction with ARHGAP10 probably changes PAK-2p34 location to cytoplasmic perinuclear region. Myristoylation changes PAK-2p34 location to the membrane.
  • Information by UniProt

Recombinant human PAK2 protein images

  • SDS-PAGE analysis of PAK2 (ab95310) and BSA

    1, 2, 3 :  ab95310 at 6, 60 and 120ug/ml

    4, 5, 6 : BSA at 5, 50, 100ug/ml

     

     

References for Recombinant human PAK2 protein (ab95310)

ab95310 has not yet been referenced specifically in any publications.

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