Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Molecular weight
      90 kDa including tags
    • Amino acids
      1 to 591

Specifications

Our Abpromise guarantee covers the use of ab96405 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    The specific activity of ab96405 was determined to be 72 nmol/min/mg.
  • Applications

    SDS-PAGE

    Functional Studies

  • Purity
    90 % .
    Purity was determined to be >90% by densitometry.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • KIAA1142
    • p21 activated kinase 4
    • p21 Cdc42/Rac1-actiated kinase 4
    • P21 protein (Cdc42/Rac) activated kinase 4
    • p21(CDKN1A) activated kinase 4
    • p21-activated kinase 4
    • PAK 4
    • PAK-4
    • Pak4
    • PAK4_HUMAN
    • Protein kinase related to S.cerevisiae STE20 effector for Cdc42Hs
    • Serine threonine kinase PAK 4
    • Serine/threonine protein kinase PAK 4
    • Serine/threonine protein kinase PAK4
    • Serine/threonine-protein kinase PAK 4
    see all
  • Function
    Activates the JNK pathway. Plays a role in the reorganization of the actin cytoskeleton and in the formation of filopodia. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates ARHGEF2.
  • Tissue specificity
    Highest expression in prostate, testis and colon.
  • Sequence similarities
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 CRIB domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated on serine residues when activated by CDC42/p21.
    Phosphorylated on tyrosine residues upon stimulation of FGFR2.
  • Information by UniProt

Images

  • Kinase Assay demonstrating specific activity of ab96405.
  • SDS-PAGE showing ab96405 at approximately 90kDa.

References

ab96405 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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