Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionQ9P286
    • SpeciesHuman
    • Molecular weight34 kDa
    • Amino acids425 to 719

Specifications

Our Abpromise guarantee covers the use of ab95311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activitySpecific Activity: 4.2 nmoles/min/µg. Analysis of enzymatic activity: 1. Different concentrations of PAK5 were incubated in a buffer containing 50 mM HEPES (pH7.5), 10 mM MgCl2, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35, and 2 µM substrate (SER/THR 20) at room temperature for 1 hour. 2. Developer solution was added to reaction and reaction was stopped after 1h of incubation at RT. 3. Fluorescence was detected using ?exc=460±40 nm and ?em=528±20 nm filters.
  • Applications

    Functional Studies

  • Purity> 90 % SDS-PAGE.

  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 10% Glycerol, 5mM DTT, 25mM Tris HCl, 150mM Sodium chloride, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • EC 2.7.11.1
    • KIAA1264
    • MGC26232
    • p21 activated kinase 7
    • p21 protein (Cdc42/Rac)-activated kinase 7
    • p21(CDKN1A) activated kinase 7
    • p21-activated kinase 5
    • p21-activated kinase 7
    • PAK 5
    • PAK 7
    • PAK-5
    • PAK-7
    • PAK5
    • PAK7
    • PAK7_HUMAN
    • Protein kinase PAK5
    • Serine/threonine protein kinase PAK 7
    • Serine/threonine-protein kinase PAK 7
    see all
  • FunctionThe activated kinase acts on a variety of targets.
  • Tissue specificityPredominantly expressed in brain.
  • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 CRIB domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated when activated by CDC42/p21.
  • Information by UniProt

Recombinant human PAK5 protein images

  • Image showing activity analysis of PAK5 (ab95311).

  • Image showing SDS-PAGE analysis of ab95311.

    1, 2, 3 : ab95311 at 6, 60 and 120 ug/ml.

References for Recombinant human PAK5 protein (ab95311)

ab95311 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"