Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionQ9NQU5
    • SpeciesHuman
    • Molecular weight34 kDa including tags
    • Amino acids385 to 680

Specifications

Our Abpromise guarantee covers the use of ab95321 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activitySpecific Activity: 2.1 nmoles/min/µg.
    Analysis of enzymatic activity: 1. Different concentration of PAK6 were incubated in a buffer containing 50 mM HEPES (pH7.5), 10 mM MgCl2, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35, and 2 µM substrate (SER/THR 20) at room temperature for 1 hour.
    2. Developer solution was added to reaction and reaction was stopped after 1h of incubation at RT.
    3. Fluorescence was detected using ?exc=460±40 nm and ?em=528±20 nm filters.
  • Applications

    Functional Studies

  • Purity> 90 % SDS-PAGE.

  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 10% Glycerol, 5mM DTT, 25mM Tris HCl, 150mM Sodium chloride, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • CDKN1A activated kinase 6
    • EC 2.7.1.37
    • p21 activated protein kinase 6
    • p21 protein (Cdc42/Rac)-activated kinase 6
    • p21(CDKN1A) activated kinase 6
    • p21-activated kinase 6
    • p21activated kinase 6
    • PAK 5
    • PAK 6
    • PAK-5
    • PAK-6
    • PAK5
    • Pak6
    • PAK6_HUMAN
    • Serine threonine protein kinase PAK 6
    • Serine/threonine protein kinase PAK 6
    • Serine/threonine protein kinase PAK6
    • Serine/threonine-protein kinase PAK 6
    see all
  • FunctionThe activated kinase acts on a variety of targets.
  • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 CRIB domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated when activated by CDC42/p21.
  • Information by UniProt

Recombinant human PAK6 protein images

  • Image shows activity analysis of ab95321.

  • Image showing SDS-PAGE analysis of ab95321.

    1, 2, 3, 4, 5 : ab95321 at 7, 14, 28, 56 and 112ug/ml.

References for Recombinant human PAK6 protein (ab95321)

ab95321 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"