Recombinant Human Parathyroid Hormone protein (ab51234)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Human
    • Sequence
      MSVSEIQLMH NLGKHLNSME RVEWLRKKLQ DVHNFVALGA PLAPRDAGSQ RPRKKEDNVL VESHEKSLGE ADKADVNVLT KAKSQ

Specifications

Our Abpromise guarantee covers the use of ab51234 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    ELISA

    Western blot

    SDS-PAGE

  • Purity
    > 95 % SDS-PAGE.
    Human Parathyroid Hormone(amino acids 1-84) was overexpressed in E. coli and purified to apparent homogeneity by using conventional column chromatography techniques.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: PBS, 2mM EDTA, pH 7.4

General Info

  • Alternative names
    • hPTH
    • Parathormone
    • Parathyrin
    • Parathyroid hormone
    • Parathyroid hormone 1
    • Prepro PTH
    • Preproparathyroid hormone
    • PTH
    • PTH1
    • PTH1 receptor
    • PTH1R
    • PTHR
    • PTHR1
    • PTHY_HUMAN
    see all
  • Function
    PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells.
  • Involvement in disease
    Defects in PTH are a cause of familial isolated hypoparathyroidism (FIH) [MIM:146200]; also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. FIH exist both as autosomal dominant and recessive forms of hypoparathyroidism.
  • Sequence similarities
    Belongs to the parathyroid hormone family.
  • Cellular localization
    Secreted.
  • Information by UniProt

Recombinant Human Parathyroid Hormone protein images

  • Standard curve for Parathyroid Hormone (Analyte: ab51234); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [A1/70] to Parathyroid Hormone (ab14493) at 0.2µg/ml and Detector Antibody Rabbit polyclonal to Parathyroid Hormone (ab53040) at 0.5µg/ml.
  • ab51234 on 15%SDS-PAGE
  • Anti-Parathyroid Hormone antibody (ab40630) at 1 µg/ml + Recombinant Human Parathyroid Hormone protein (ab51234) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 4 minutes

References for Recombinant Human Parathyroid Hormone protein (ab51234)

ab51234 has not yet been referenced specifically in any publications.

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