Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150.
Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR. Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites. S-nitrosylated, leading to inhibit transcription regulation activity.
SDS-PAGE - Recombinant Human PARP1 protein (ab75605)
Lane A: unmodified PARP1 LANE B: automodified PARP1 Lane B shows a smear of staining above MW 113kDa indicating polyADP-ribosylated PARP1 modified to various extents. A significant amount of automodified PARP1 did not enter the gel (top arrow). This image shows that most of the PARP1 will be at the top of the gel with a smear running up from 113kDa indicating PARP1 is automodified to various extents. There is little or no native PARP1 at 113kDa.
Western blot - Recombinant Human PARP1 protein (ab75605)
All lanes : Anti-PARP1 antibody at 5 µg/ml
Lane 1 : PARP1 - unmodified Lane 2 : Recombinant Human PARP1 protein (ab75605)