Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Species
      Human

Specifications

Our Abpromise guarantee covers the use of ab79638 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Specific Activity: 207.5 U/mg.
  • Applications

    Functional Studies

    SDS-PAGE

  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 50% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • ADP ribosyltransferase (NAD+; poly (ADP ribose)
    • ADP ribosyltransferase diphtheria toxin like 3
    • ADPRT-3
    • ADPRT3
    • ADPRTL2
    • ADPRTL3
    • ARTD3
    • hPARP 3
    • hPARP-3
    • hPARP3
    • IRT 1
    • IRT1
    • NAD(+) ADP ribosyltransferase 3
    • NAD(+) ADP-ribosyltransferase 3
    • NAD+ ADP ribosyltransferase 3
    • pADPRT 3
    • pADPRT-3
    • pADPRT3
    • PARP 3
    • PARP-3
    • PARP3
    • PARP3_HUMAN
    • Poly (ADP ribose) polymerase family, member 3
    • Poly (ADP ribose) synthetase 3
    • Poly [ADP-ribose] polymerase 3
    • Poly[ADP ribose] synthetase 3
    • Poly[ADP-ribose] synthase 3
    see all
  • Function
    Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. May link the DNA damage surveillance network to the mitotic fidelity checkpoint. Negatively influences the G1/S cell cycle progression without interfering with centrosome duplication. Binds DNA. May be involved in the regulation of PRC2 and PRC3 complex-dependent gene silencing.
  • Tissue specificity
    Widely expressed; the highest levels are in the kidney, skeletal muscle, liver, heart and spleen; also detected in pancreas, lung, placenta, brain, leukocytes, colon, small intestine, ovary, testis, prostate and thymus.
  • Sequence similarities
    Contains 1 PARP alpha-helical domain.
    Contains 1 PARP catalytic domain.
  • Domain
    According to PubMed:10329013, the N-terminal domain (54 amino acids) of isoform 2 is responsible for its centrosomal localization. The C-terminal region contains the catalytic domain.
  • Post-translational
    modifications
    Auto-poly(ADP)-ribosylation.
  • Cellular localization
    Nucleus. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > centrosome > centriole. Core component of the centrosome. Preferentially localized to the daughter centriole throughout the cell cycle. According to PubMed:16924674, it is almost exclusively localized in the nucleus and appears in numerous small foci and a small number of larger foci whereas a centrosomal location has not been detected.
  • Information by UniProt

Images

  • PARP assay - Specific activity 207.5 U/mg
  • ab79638 on SDS-PAGE, Molecular Weight 87 kDa
    10% SDS-PAGE, Coomassie staining
    Lane 1: ab79638 6µg
    Lane 2: Protein marker

References

ab79638 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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