Overview

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • SpeciesHuman

Associated products

Specifications

Our Abpromise guarantee covers the use of ab79638 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activitySpecific Activity: 207.5 U/mg.
  • Applications

    Functional Studies

    SDS-PAGE

  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 50% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • ADP ribosyltransferase (NAD+; poly (ADP ribose)
    • ADP ribosyltransferase diphtheria toxin like 3
    • ADPRT-3
    • ADPRT3
    • ADPRTL2
    • ADPRTL3
    • ARTD3
    • hPARP 3
    • hPARP-3
    • hPARP3
    • IRT 1
    • IRT1
    • NAD(+) ADP ribosyltransferase 3
    • NAD(+) ADP-ribosyltransferase 3
    • NAD+ ADP ribosyltransferase 3
    • pADPRT 3
    • pADPRT-3
    • pADPRT3
    • PARP 3
    • PARP-3
    • PARP3
    • PARP3_HUMAN
    • Poly (ADP ribose) polymerase family, member 3
    • Poly (ADP ribose) synthetase 3
    • Poly [ADP-ribose] polymerase 3
    • Poly[ADP ribose] synthetase 3
    • Poly[ADP-ribose] synthase 3
    see all
  • FunctionInvolved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. May link the DNA damage surveillance network to the mitotic fidelity checkpoint. Negatively influences the G1/S cell cycle progression without interfering with centrosome duplication. Binds DNA. May be involved in the regulation of PRC2 and PRC3 complex-dependent gene silencing.
  • Tissue specificityWidely expressed; the highest levels are in the kidney, skeletal muscle, liver, heart and spleen; also detected in pancreas, lung, placenta, brain, leukocytes, colon, small intestine, ovary, testis, prostate and thymus.
  • Sequence similaritiesContains 1 PARP alpha-helical domain.
    Contains 1 PARP catalytic domain.
  • DomainAccording to PubMed:10329013, the N-terminal domain (54 amino acids) of isoform 2 is responsible for its centrosomal localization. The C-terminal region contains the catalytic domain.
  • Post-translational
    modifications
    Auto-poly(ADP)-ribosylation.
  • Cellular localizationNucleus. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > centrosome > centriole. Core component of the centrosome. Preferentially localized to the daughter centriole throughout the cell cycle. According to PubMed:16924674, it is almost exclusively localized in the nucleus and appears in numerous small foci and a small number of larger foci whereas a centrosomal location has not been detected.
  • Information by UniProt

Recombinant human PARP3 protein images

  • PARP assay - Specific activity 207.5 U/mg
  • ab79638 on SDS-PAGE, Molecular Weight 87 kDa
    10% SDS-PAGE, Coomassie staining
    Lane 1: ab79638 6µg
    Lane 2: Protein marker

References for Recombinant human PARP3 protein (ab79638)

ab79638 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab79638.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"