Recombinant Human PC4 (mutated F77) protein (ab82047)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Human

Associated products

Specifications

Our Abpromise guarantee covers the use of ab82047 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

    EMSA

  • Purity
    > 95 % SDS-PAGE.
    ab82047 is purified by conventional chromatography.
  • Form
    Liquid
  • Additional notes


    A single amino acid change at position 77 (F to P) abolishes both ds- and ss-DNA binding activity.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 20% Glycerol, 20mM Tris Cl, 100mM Potassium chloride, 1mM DTT, 0.2mM EDTA, pH 8.0

General Info

  • Alternative names
    • Activated RNA polymerase II transcriptional coactivator p15
    • Interferon related developmental regulator 1
    • MGC102747
    • p14
    • P15
    • PC4
    • PC4 LSB
    • Positive cofactor 4
    • RPO2TC1
    • Sub1
    • SUB1 homolog
    • TCP4_HUMAN
    see all
  • Function
    General coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. May be involved in stabilizing the multiprotein transcription complex. Binds single-stranded DNA. Also binds, in vitro, non-specifically to double-stranded DNA (ds DNA).
  • Sequence similarities
    Belongs to the transcriptional coactivator PC4 family.
  • Post-translational
    modifications
    Activity is controlled by protein kinases that target the regulatory region. Phosphorylation inactivates both ds DNA-binding and cofactor function, but does not affect binding to ssDNA. Seems to be phosphorylated in vivo by CK2 in at least 7 sites in the N-terminal Ser-rich region.
  • Cellular localization
    Nucleus.
  • Information by UniProt

References

ab82047 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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