Recombinant Human PCDH12 protein (ab182818)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant Human PCDH12 protein -
Purity
> 90 % SDS-PAGE.
ab182818 was expressed in E.coli as inclusion bodies, refolded using a unique “temperature shift inclusion body refolding” technology and chromatographically purified. -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MASMTGGQQMGRGHHHHHHGNLYFQGGEFQEVTTLTVKYQVSEEVPSGTV IGKLSQELGREERRRQAGAAFQVLQLPQALPIQVDSEEGLLSTGRRLDRE QLCRQWDPCLVSFDVLATGDLALIHVEIQVLDINDHQPRFPKGEQELEIS ESASLRTRIPLDRALDPDTGPNTLHTYTLSPSEHFALDVIVGPDETKHAE LIVVKELDREIHSFFDLVLTAYDNGNPPKSGTSLVKVNVLDSNDNSPAFA ESSLALEIQEDAAPGTLLIKLTATDPDQGPNGEVEFFLSKHMPPEVLDTF SIDAKTGQVILRRPLDYEKNPAYEVDVQARDLGPNPIPAHCKVLIKVLDV NDNIPSIHVTWASQPSLVSEALPKDSFIALVMADDLDSGNNGLVHCWLSQ ELGHFRLKRTNGNTYMLLTNATLDREQWPKYTLTLLAQDQGLQPLSAKKQ LSIQISDINDNAPVFEKSRYEVSTRENNLPSLHLITIKAHDADLGINGKV SYRIQDSPVAHLVAIDSNTGEVTAQRSLNYEEMAGFEFQVIAEDSGQPML ASSVSVWVSLLDANDNAPEVVQPVLSDGKASLSVLVNASTGHLLVPIETP NGLGPAGTDTPPLATHSSRPFLLTTIVARDADSGANGEPLYSIRSGNEAH LFILNPHTGQLFVNVTNASSLIGSEWELEIVVEDQGSPPLQTRALLRVMF VTSVDHLRDSARKPGALSMSMLT -
Predicted molecular weight
79 kDa including tags -
Amino acids
25 to 718 -
Additional sequence information
Constructed with codon optimization and expressed with a small T7-His-TEV cleavage site tag (29aa) fusion at its N-terminal (NP_057664).
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab182818 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -80°C.
pH: 8.00
Constituent: 0.32% Tris HCl
Contains NACl, EDTA, KCl, arginine, DTT and glycerol
General Info
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Alternative names
- PCD12_HUMAN
- Pcdh12
- Protocadherin 12
see all -
Function
Cellular adhesion molecule that may play an important role in cell-cell interactions at interendothelial junctions. Promotes homotypic calcium dependent aggregation and adhesion and clusters at intercellular junctions. Unable to bind to catenins, weakly associates with the cytoskeleton. -
Tissue specificity
Expressed in highly vascularized tissues including the heart and placenta, but most tissues contain a low level of expression. Prominent expression in the spleen. -
Sequence similarities
Contains 6 cadherin domains. -
Cellular localization
Cell membrane. Cell junction. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab182818 has not yet been referenced specifically in any publications.