Recombinant Human PDE12 protein (ab107695)

Overview

  • Product nameRecombinant Human PDE12 protein
  • Protein lengthFull length protein

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • AccessionQ6L8Q7
    • SpeciesHuman
    • Molecular weight100 kDa including tags
    • Amino acids1 to 609

Specifications

Our Abpromise guarantee covers the use of ab107695 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Purity> 90 % SDS-PAGE.
    Purity determined by densitometry
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Sodium chloride, 50mM Tris HCl, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

General Info

  • Alternative names
    • 2 PDE
    • 2' 5' phosphodiesterase 12
    • 2' phosphodiesterase
    • 2',5'-phosphodiesterase 12
    • 2'-PDE
    • 2-PDE
    • 2PDE
    • DKFZp667B1218
    • Pde12
    • PDE12_HUMAN
    • Phosphodiesterase 12
    see all
  • FunctionEnzyme that cleaves 2',5'-phosphodiester bond linking adenosines of the 5'-triphosphorylated oligoadenylates, triphosphorylated oligoadenylates referred as 2-5A modulates the 2-5A system. This enzyme degraded triphosphorylated 2-5A to produce AMP and ATP. Also cleaves 3',5'-phosphodiester bond of oligoadenylates. Play a role as a negative regulator of the The 2-5A system that is one of the major pathways for antiviral and antitumor functions induced by interferons (IFNs). Suppression of this enzyme induces reduction of viral replication in Hela cells, thus counteracting the antiviral pathway probably by inhibiting the 2-5A system.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the CCR4/nocturin family.
  • Information by UniProt

Recombinant Human PDE12 protein images

  • SDS page analysis of ab107695

References for Recombinant Human PDE12 protein (ab107695)

ab107695 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your patience and for your feedback. I am very sorry to hear that the new protein does also not show enough activity for being used in an activity assay. In regards to use the whole cell extract, I am not very optimistic to be honest. I a...

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Thank you for your patience. I checked with the laboratory and our supply chain department, and we estimate to be able to deliver the product to you either Wednesday or Thursday of next week. I will be happy to send you another update next Monday. Plea...

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Application Other

Review text: Dilutions of PDE12 (1 to 10ml) were incubated for 2h with 20µl of PDE12 assay substrate, an analogue of 2-5A dual labelled with Fam and Dabcyl. Active PDE12 is expected to cleave 2-5A molecules releasing the quencher from the Fluorophore, allowing it to fluoresce which is detected by the Flo-Star Optima (Ex 485nm and Em 520nm). Delta fluorescence (T120min-Tzero min) is used to calculate PDE12 % activity using cell lysate as positive control for PDE12.

Unfortunately there was no detectable fluorescence (Tzero and T120 were almost the same) observed after incubating PDE12 (ab107695) for 2h suggesting the protein under the storage conditions provided was not active.
Sample: Human Purified protein

Primary antibody (in addition to 'PDE12 protein')
Primary antibody: None used

Secondary antibody
Secondary antibody: None used

Additional data
Abcam response: Accordingly to these results, this protein preparation has not conserved its functional activity. We thank the researcher to have participated in our testing offer. Unfortunately we have to conclude that this protein in ists current formulation seems not suitable for functional tests.
Username

Dr. fouzia sadiq

Verified customer

Submitted Sep 16 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"