Overview

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • AccessionQ08499-2
    • SpeciesHuman
    • Molecular weight82 kDa including tags
    • Amino acids225 to 673

Specifications

Our Abpromise guarantee covers the use of ab125534 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityThe specific activity of ab125534 was determined to be 1145 - 1200 nmol/min/mg.
  • Applications

    Functional Studies

    SDS-PAGE

  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 25% Glycerol, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • cAMP specific 3',5' cyclic phosphodiesterase 4D
    • DKFZp686M11213
    • 5''-cyclic phosphodiesterase 4D
    • cAMP specific phosphodiesterase 4D
    • cAMP specific phosphodiesterase PDE4D6
    • cAMP-specific 3''
    • DPDE3
    • Dunce like phosphodiesterase E3
    • FLJ97311
    • HSPDE4D
    • PDE43
    • Pde4d
    • PDE4D_HUMAN
    • PDE4DN2
    • Phosphodiesterase 4D cAMP specific
    • Phosphodiesterase 4D cAMP specific (dunce (Drosophila) homolog phosphodiesterase E3)
    • Phosphodiesterase 4D cAMP specific (phosphodiesterase E3 dunce homolog Drosophila)
    • Phosphodiesterase 4D, cAMP specific (dunce)
    • STRK1
    see all
  • FunctionHydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
  • Tissue specificityWidespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle.
  • PathwayPurine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
  • Involvement in diseaseNote=Genetic variations in PDE4D might be associated with susceptibility to stroke. PubMed:17006457 states that association with stroke has to be considered with caution.
  • Sequence similaritiesBelongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.
  • Post-translational
    modifications
    Isoform 3 and isoform 7 are activated by phosphorylation (in vitro), but not isoform 6. Isoform N3 and isoform 12 are phosphorylated on Ser-49, Ser-51, Ser-55 and Ser-59.
  • Cellular localizationCytoplasm. Membrane. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Found in the soluble fraction, associated with membranes, and associated with the cytoskeleton and the centrosome.
  • Information by UniProt

Recombinant human PDE4D protein images

  • SDS-PAGE analysis of ab125534.
  • Sample Phosphodiesterase Assay showing the specific activity of ab125534 as 1200 nmol/min/mg.

References for Recombinant human PDE4D protein (ab125534)

ab125534 has not yet been referenced specifically in any publications.

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