Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Molecular weight
      82 kDa including tags
    • Amino acids
      225 to 673

Specifications

Our Abpromise guarantee covers the use of ab125534 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    The specific activity of ab125534 was determined to be 1145 - 1200 nmol/min/mg.
  • Applications

    Functional Studies

    SDS-PAGE

  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 25% Glycerol, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • cAMP specific 3',5' cyclic phosphodiesterase 4D
    • DKFZp686M11213
    • 5''-cyclic phosphodiesterase 4D
    • cAMP specific phosphodiesterase 4D
    • cAMP specific phosphodiesterase PDE4D6
    • cAMP-specific 3''
    • DPDE3
    • Dunce like phosphodiesterase E3
    • FLJ97311
    • HSPDE4D
    • PDE43
    • Pde4d
    • PDE4D_HUMAN
    • PDE4DN2
    • Phosphodiesterase 4D cAMP specific
    • Phosphodiesterase 4D cAMP specific (dunce (Drosophila) homolog phosphodiesterase E3)
    • Phosphodiesterase 4D cAMP specific (phosphodiesterase E3 dunce homolog Drosophila)
    • Phosphodiesterase 4D, cAMP specific (dunce)
    • STRK1
    see all
  • Function
    Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
  • Tissue specificity
    Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle.
  • Pathway
    Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
  • Involvement in disease
    Note=Genetic variations in PDE4D might be associated with susceptibility to stroke. PubMed:17006457 states that association with stroke has to be considered with caution.
  • Sequence similarities
    Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.
  • Post-translational
    modifications
    Isoform 3 and isoform 7 are activated by phosphorylation (in vitro), but not isoform 6. Isoform N3 and isoform 12 are phosphorylated on Ser-49, Ser-51, Ser-55 and Ser-59.
  • Cellular localization
    Cytoplasm. Membrane. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Found in the soluble fraction, associated with membranes, and associated with the cytoskeleton and the centrosome.
  • Information by UniProt

Recombinant human PDE4D protein images

  • SDS-PAGE analysis of ab125534.
  • Sample Phosphodiesterase Assay showing the specific activity of ab125534 as 1200 nmol/min/mg.

References for Recombinant human PDE4D protein (ab125534)

ab125534 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab125534.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up