Overview

  • Product nameRecombinant human PDE4D3 protein
  • Protein lengthFull length protein

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • SpeciesHuman

Specifications

Our Abpromise guarantee covers the use of ab80332 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activitySpecific Activity: 3703 pmole/min/µg.
  • Applications

    Functional Studies

    SDS-PAGE

    Western blot

  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 30% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • DPDE3
    • hPDE4D3
    • HSPDE4D
    • PDE43
    • PDE4D
    • PDE4DN2
    • Phosphodiesterase 4D
    • Phosphodiesterase 4D cAMP-specific
    • STRK1
    see all
  • RelevanceCyclic AMP-dependent phosphodiesterase type D (PDE4D) family is comprised of 5 variants (PDE4D1, D2, D3, D4 and D5). One or more PDE4D subtype-variants are ubiquitously present in all mammalian cells. In CNS all five PDE4D subtype-variants are expressed in varying ratios and their activity is regulated in tandem with GPCRs stimulation. Peripheral tissues also exhibit differential expression of PDE4D variants. PDE4D1/D2 mRNA levels rise in response to an increase in cAMP. Short-term regulation of PDE4D variants involved PKA, MAP kinases and Erk2 phosphorylation that results in rapid change in their enzymatic activities. Other regulatory mechanism involved protein-protein interactions with cytoskeletal scaffolding proteins.
  • Cellular localizationCell Membrane and Cytoplasmic

Recombinant human PDE4D3 protein images

  • 10% SDS-PAGE, Coomassie staining.
    Lane 1: ab80332, 4µg.
    Lane 2: Protein marker.
  • Image showing specific activity of ab80332.
  • Anti-PDE4D3 antibody (ab14614) at 1/500 dilution + Recombinant human PDE4D3 protein (ab80332) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 8 minutes

References for Recombinant human PDE4D3 protein (ab80332)

ab80332 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab80332.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"