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- SourceBaculovirus infected Sf9 cells
- Amino Acid Sequence
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- Biological activity
Specific Activity: ≥ 3,100 pmole/min/µg
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Preparation and Storage
- Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 50% Glycerol, 0.05% Tween 20, 5mM Glutathione, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- 5''-cyclic phosphodiesterase
- CGB PDE
- FunctionPlays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP.
- Tissue specificityExpressed in aortic smooth muscle cells, heart, placenta, skeletal muscle and pancreas and, to a much lesser extent, in brain, liver and lung.
- PathwayPurine metabolism; 3',5'-cyclic GMP degradation; GMP from 3',5'-cyclic GMP: step 1/1.
- Sequence similaritiesBelongs to the cyclic nucleotide phosphodiesterase family.
Contains 2 GAF domains.
- DomainComposed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain which contains two homologous allosteric cGMP-binding regions, A and B.
modificationsPhosphorylation is regulated by binding of cGMP to the two allosteric sites.
Information by UniProt
References for Recombinant human PDE5A protein (ab56614)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"