Overview

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • AccessionQ13946
    • SpeciesHuman
    • Molecular weight64 kDa including tags
    • Amino acids1 to 456

Specifications

Our Abpromise guarantee covers the use of ab125786 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityThe specific activity of ab125786 was determined to be 166 nmol/min/mg.
  • Applications

    Functional Studies

    SDS-PAGE

  • Purity> 85 % Densitometry.

  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.002% PMSF, 0.4% DTT, 0.79% Tris HCl, 25% Glycerol, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • 5''-cyclic phosphodiesterase 7A
    • HCP 1
    • HCP1
    • High affinity cAMP specific 3'5' cyclic phosphodiesterase 7A
    • High affinity cAMP-specific 3''
    • P2A
    • PDE 7
    • PDE 7A
    • PDE1/PDE2 yeast human complement of
    • PDE7
    • PDE71
    • PDE7A
    • PDE7A_HUMAN
    • Phosphodiesterase 7A
    • Phosphodiesterase isozyme 7
    • Phosphodiesterase7A
    • Rolipram insensitive phosphodiesterase type 7
    • TM 22
    • TM22
    see all
  • FunctionHydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May have a role in muscle signal transduction.
  • Tissue specificityPDE7A1 is found at high levels in skeletal muscle and at low levels in a variety of tissues including brain and heart. It is expressed as well in two T-cell lines. PDE7A2 is found abundantly in skeletal muscle and at low levels in heart.
  • PathwayPurine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
  • Sequence similaritiesBelongs to the cyclic nucleotide phosphodiesterase family. PDE7 subfamily.
  • Developmental stageDevelopmentally regulated. PDE7A1 and PDE7A2 are found in several fetal tissues, expression is reduced throughout development. It persists strongly only in adult skeletal muscle.
  • DomainComposed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain.
  • Cellular localizationCytoplasm > cytosol. PDE7A1 (57 kDa) is located mostly to soluble cellular fractions and Cytoplasm. PDE7A2 (50 kDa) is located to particulate cellular fractions.
  • Information by UniProt

Recombinant human PDE7A protein images

  • SDS-PAGE analysis of ab125786.
  • Sample assay showing the specific activity of ab125786 to be 166 nmol/min/mg.

References for Recombinant human PDE7A protein (ab125786)

ab125786 has not yet been referenced specifically in any publications.

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