Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSSNSL NILDDDYNGQ AKCMLEKVGN WNFDIFLFDR LTNGNSLVSL TFHLFSLHGL IEYFHLDMMK LRRFLVMIQE DYHSQNPYHN AVHAADVTQA MHCYLKEPKL ANSVTPWDIL LSLIAAATHD LDHPGVNQPF LIKTNHYLAT LYKNTSVLEN HHWRSAVGLL RESGLFSHLP LESRQQMETQ IGALILATDI SRQNEYLSLF RSHLDRGDLC LEDTRHRHLV LQMALKCADI CNPCRTWELS KQWSEKVTEE FFHQGDIEKK YHLGVSPLCD RHTESIANIQ IGFMTYLVEP LFTEWARFSN TRLSQTMLGH VGLNKASWKG LQREQSSSED TDAAFELNSQ LLPQENRLS
    • Molecular weight
      64 kDa including tags
    • Amino acids
      104 to 456
    • Tags
      GST tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab125786 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    The specific activity of ab125786 was determined to be 166 nmol/min/mg.
  • Applications

    Functional Studies

    SDS-PAGE

  • Purity
    > 85 % Densitometry.

  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.002% PMSF, 0.4% DTT, 0.79% Tris HCl, 25% Glycerol, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • 5''-cyclic phosphodiesterase 7A
    • HCP 1
    • HCP1
    • High affinity cAMP specific 3'5' cyclic phosphodiesterase 7A
    • High affinity cAMP-specific 3''
    • P2A
    • PDE 7
    • PDE 7A
    • PDE1/PDE2 yeast human complement of
    • PDE7
    • PDE71
    • PDE7A
    • PDE7A_HUMAN
    • Phosphodiesterase 7A
    • Phosphodiesterase isozyme 7
    • Phosphodiesterase7A
    • Rolipram insensitive phosphodiesterase type 7
    • TM 22
    • TM22
    see all
  • Function
    Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May have a role in muscle signal transduction.
  • Tissue specificity
    PDE7A1 is found at high levels in skeletal muscle and at low levels in a variety of tissues including brain and heart. It is expressed as well in two T-cell lines. PDE7A2 is found abundantly in skeletal muscle and at low levels in heart.
  • Pathway
    Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
  • Sequence similarities
    Belongs to the cyclic nucleotide phosphodiesterase family. PDE7 subfamily.
  • Developmental stage
    Developmentally regulated. PDE7A1 and PDE7A2 are found in several fetal tissues, expression is reduced throughout development. It persists strongly only in adult skeletal muscle.
  • Domain
    Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain.
  • Cellular localization
    Cytoplasm > cytosol. PDE7A1 (57 kDa) is located mostly to soluble cellular fractions and Cytoplasm. PDE7A2 (50 kDa) is located to particulate cellular fractions.
  • Information by UniProt

Images

  • SDS-PAGE analysis of ab125786.
  • Sample assay showing the specific activity of ab125786 to be 166 nmol/min/mg.

References

ab125786 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab125786.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up