Overview

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • AccessionO76083-2
    • SpeciesHuman
    • Molecular weight86 kDa including tags
    • Amino acids1 to 533

Specifications

Our Abpromise guarantee covers the use of ab125666 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityThe specific activity of ab125666 was determined to be 53 - 60 nmol/min/mg.
  • Applications

    Functional Studies

    SDS-PAGE

  • Purity> 90 % Densitometry.
    Purity was determined to be >90% by densitometry.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • 5''-cyclic phosphodiesterase 9A
    • cGMP specific 3' 5' cyclic phosphodiesterase type 9
    • FLJ90181
    • High affinity cGMP-specific 3''
    • High-affinity cGMP-specific 3'5'-cyclic phosphodiesterase 9A
    • HSPDE9A2
    • OTTHUMP00000109399
    • PDE 9A
    • Pde9a
    • PDE9A_HUMAN
    • Phosphodiesterase 9A
    • phosphodiesterase PDE9A21
    see all
  • FunctionHydrolyzes the second messenger cGMP, which is a key regulator of many important physiological processes.
  • Tissue specificityExpressed in all tissues examined (testis, brain, small intestine, skeletal muscle, heart, lung, thymus, spleen, placenta, kidney, liver, pancreas, ovary and prostate) except blood. Highest levels in brain, heart, kidney, spleen, prostate and colon. Isoform PDE9A12 is found in prostate.
  • Sequence similaritiesBelongs to the cyclic nucleotide phosphodiesterase family. PDE9 subfamily.
  • DomainComposed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain.
  • Cellular localizationCytoplasm. Endoplasmic reticulum; Cell projection > ruffle membrane. Cytoplasm > perinuclear region and Cell projection > ruffle membrane. Cytoplasm > perinuclear region. Golgi apparatus. Endoplasmic reticulum.
  • Information by UniProt

Recombinant human PDE9A protein images

  • SDS-PAGE analysis of ab125666.
  • Sample Phosphodiesterase Assay showing the specific activity of ab125666 to be 60 nmol/min/mg.

References for Recombinant human PDE9A protein (ab125666)

ab125666 has not yet been referenced specifically in any publications.

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