Recombinant human PDGF AB protein (ab73228)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Human
    • Sequence
      The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ser-Ile-Glu-Glu- (A chain) and Met-Ser-Leu-Gly-Ser (B chain).

Specifications

Our Abpromise guarantee covers the use of ab73228 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Specific Activity of 1 MIU/mg.
  • Applications

    SDS-PAGE

    Functional Studies

  • Purity
    > 95 % SDS-PAGE.
    This protein was purified by proprietary chromatographic techniques. Purity greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
  • Form
    Lyophilised
  • Additional notes
    For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    No additives

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    It is recommended to reconstitute the lyophilized PDGF AB in sterile 18Mohm/cm H2O at not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

General Info

  • Alternative names
    • becaplermin
    • c-sis
    • GDGF
    • ODGF
    • Oncogene SIS
    • PDGF A chain
    • PDGF B chain
    • PDGF subunit A
    • PDGF subunit B
    • PDGF1
    • PDGF2
    • PDGFA
    • PDGFB
    • platelet-derived growth factor 2
    • platelet-derived growth factor A chain
    • platelet-derived growth factor alpha chain
    • platelet-derived growth factor alpha isoform 2 preproprotein
    • platelet-derived growth factor alpha polypeptide
    • platelet-derived growth factor B chain
    • platelet-derived growth factor beta polypeptide
    • platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog)
    • platelet-derived growth factor subunit A
    • platelet-derived growth factor subunit B
    • platelet-derived growth factor, beta polypeptide (oncogene SIS)
    • proto-oncogene c-Sis
    • Simian sarcoma viral oncogene homolog
    • SIS
    • SSV
    • V-SIS platelet-derived growth factor beta polypeptide
    see all
  • Relevance
    Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle and connective tissue. PDGF, which is composed of a dimer of two chains joined by disulphide bonds, termed the A chain and B chain, can be present as AA or BB homodimers or as an AB heterodimer. Human PDGF-AB is a 25.5 kDa heterodimer protein consisting of a 13.3 kDa A chain and a 12.2 kDa B chain.
  • Cellular localization
    Secreted

References

ab73228 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab73228.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up