The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Immobilized Human PDGF-BB, His Tag (ab221402) at 5 µg/mL (100 µl/well) can bind Human PDGF R beta with a linear range of 0.02-1.2 µg/mL.
< 1.000 Eu/µg
>95% by SDS-PAGE .
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.40 Constituent: 5% Trehalose
Acetonitrile and TFA.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
It is recommended to reconstitute the lyophilized product in sterile deionized water to a final concentration of 1 mg/mL. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.