Recombinant human PDGF CC protein (ab84066)

Overview

  • Product nameRecombinant human PDGF CC protein
  • Protein lengthProtein fragment

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesHuman
    • SequenceMVVDLNLLTE EVRLYSCTPR NFSVSIREEL KRTDTIFWPG CLLVKRCGGN CACCLHNCNE CQCVPSKVTK KYHEVLQLRP KTGVRGLHKS LTDVALEHHE ECDCVCRGST GG

Specifications

Our Abpromise guarantee covers the use of ab84066 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityBiological Activity : Determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells. The expected ED50 for this effect is 15-20 ng/ml.
  • Applications

    SDS-PAGE

    Functional Studies

  • Endotoxin level< 0.100 Eu/µg
  • Purity> 95 % SDS-PAGE.
    Greater than 98% by SDS-PAGE gel and HPLC analyses.
  • FormLyophilised
  • Additional notesEndotoxin level is less than 0.1 ng per µg (1EU/µg).
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • ReconstitutionReconstituted PDGF CC is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. Avoid repeated freeze/thaw cycles.

General Info

  • Alternative names
    • FALLOTEIN
    • PDGF C
    • PDGFC
    • Platelet derived growth factor
    • platelet-derived growth factor C
    • SCDGF
    • secretory growth factor-like protein
    • Spinal cord-derived growth factor
    • VEGF E
    see all
  • RelevanceThe platelet-derived growth factor (PDGF) family of heparin-binding growth factors consists of five known members, denoted PDGF-AA, PDGF-BB, PDGF-AB, PDGF-CC and PDGF-DD. The mature and active form of these proteins, an anti-parallel disulfide-linked dimer of two 12-14 kDa polypeptide chains, is obtained through proteolytic processing of biologically inactive precursor proteins, which contain an N-terminal CUB domain and a PDGF/VEGF homologous domain. The PDGFs interact with two related protein tyrosine kinase receptors, PDGFR-alpha and PDGFR-beta, and are potent mitogens for a variety of cell types, including smooth muscle cells, connective tissue cells, bone and cartilage cells, and certain tumor cells. They play an important role in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubules epithelial cell development. Mature PDGFs are stored in platelet alpha-granules and are released upon platelet activation. PDGF-AA, -AB, -BB and –CC signal primarily through the PDGF R alpha receptor, whereas PDGF-DD interacts almost exclusively with the PDGF R beta receptor.
  • Cellular localizationCytoplasmic, Nuclear, Secreted and Cytoplasmic granule

References for Recombinant human PDGF CC protein (ab84066)

ab84066 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting Abcam regarding ab84066. I have confirmed the following reconstitution instructions with the lab: - Centrifuge the vial prior to opening. - Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. - T...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"