Recombinant human PDGFAA + PDGFBB protein (ab61903)

Overview

  • Product nameRecombinant human PDGFAA + PDGFBB protein
  • Protein lengthFull length protein

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesHuman
    • Sequencealpha chain:-MSIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATTSLN PDYREEDTGR PRESGKKRKR KRLKPT beta chain:- MSLGSLTIAE PAMIAECKTR TEVFEISRRL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRPTQVQLRP VQVRKIGIVR KKPIFKKATV TLGDHLACKC ETVAAARPVT

Specifications

Our Abpromise guarantee covers the use of ab61903 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityBiological Activity: The activity is determined by the dose-dependent proliferation of mouse 3T3 indicator cells and is typically less than 1 ng/mL.
  • Applications

    SDS-PAGE

    Functional Studies

  • Purity> 95 % SDS-PAGE.
    Purity greater than 98% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.
  • FormLyophilised
  • Additional notesIt is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 10mM Acetic acid

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • ReconstitutionIt is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.

General Info

  • Alternative names
    • becaplermin
    • c-sis
    • FLJ12858
    • PDGF 1
    • PDGF 2
    • PDGF B
    • PDGF B chain
    • PDGF subunit B
    • PDGF-2
    • Pdgfb
    • PDGFB_HUMAN
    • Platelet derived growth factor beta polypeptide
    • platelet-derived growth factor 2
    • Platelet-derived growth factor B chain
    • Platelet-derived growth factor beta polypeptide
    • Platelet-derived growth factor subunit B
    • Platelet-derived growth factor, beta polypeptide (oncogene SIS)
    • Proto-oncogene c-Sis
    • SIS
    • SSV
    see all
  • FunctionPlatelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. Binding of this growth factor to its receptor elicits a variety of cellular responses. It is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heals the wound.
  • Tissue specificityExpressed at high levels in the heart, brain (sustantia nigra), placenta and fetal kidney. Expressed at moderate levels in the brain (hippocampus), skeletal muscle, kidney and lung.
  • Involvement in diseaseNote=A chromosomal aberration involving PDGFB is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGF.
  • Sequence similaritiesBelongs to the PDGF/VEGF growth factor family.
  • Cellular localizationSecreted.
  • Information by UniProt

Recombinant human PDGFAA + PDGFBB protein images

  • ab61903 used in Western Blot. Figure: 1 ug in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% Tris-Glycine gel stained with Coomassie Blue. Human PDGF-AB is predicted to be a disulfide linked heterodimer with a predicted MW of 25.5 kDa.

References for Recombinant human PDGFAA + PDGFBB protein (ab61903)

ab61903 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"