Recombinant Human PDK2 protein (ab110354)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionQ15119
    • SpeciesHuman
    • Amino acids16 to 407
    • TagsHis tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab110354 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Sandwich ELISA

    Functional Studies

  • Purity> 90 % Purified via His tag.

  • FormLiquid
  • Additional notes

     

    PDH Kinases (PDKs) deactivate PDH by reversible phosphorylation. In humans and rodents, 4 PDK's are present in an organ-dependent manner. In all cases, PDK's are physically part of the PDH complex (binding via lipoyl domain 2 of E2), and they phosphorylate the E1 subunit at 3 specific serine residues. PDH phosphatases remove the phosphorylation and restore activity of PDH.

    Product was previously marketed under the MitoSciences sub-brand.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.08% DTT, 0.14% Sodium phosphate, 1.46% Sodium chloride, 30% Glycerol, 0.015% EDTA, 0.05% Triton-X-100

General Info

  • Alternative names
    • [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2
    • mitochondrial
    • PDHK2
    • PDK2
    • PDK2_HUMAN
    • Pyruvate dehydrogenase kinase isoform 2
    • Pyruvate dehydrogenase kinase, isozyme 2
    • Pyruvate dehydrogenase lipoamide kinase isozyme 2, mitochondrial
    see all
  • FunctionInhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
  • Tissue specificityExpressed in many tissues, with the highest level in heart and skeletal muscle, intermediate levels in brain, kidney, pancreas and liver, and low levels in placenta and lung.
  • Sequence similaritiesBelongs to the PDK/BCKDK protein kinase family.
    Contains 1 histidine kinase domain.
  • Cellular localizationMitochondrion matrix.
  • Information by UniProt

References for Recombinant Human PDK2 protein (ab110354)

ab110354 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry.

I can confirm that these proteins have been used in functional assays in the following publications using Pyruvate-dehydrogenase-PDH-Enzyme-Activity-Microplate-Assay-Kit-ab109902:

http://www.abcam.com/i...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"