Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Molecular weight
      71 kDa
    • Amino acids
      1 to 411
    • Tags
      GST tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab125560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    The specific activity of ab125560 is 20 nmol/min/mg.
  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

  • Purity
    > 90 % Densitometry.
    ab125560 is >90% pure.
  • Form
    Liquid
  • Additional notes

    ab125602 (Human Pyruvate Dehydrogenase E1-alpha subunit full length protein) can be utilized as a substrate for assessing Kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4
    • FLJ40832
    • mitochondrial
    • Pdk4
    • PDK4_HUMAN
    • Pyruvate dehydrogenase [lipoamide] kinase isozyme 4 mitochondrial
    • Pyruvate dehydrogenase kinase 4
    • Pyruvate dehydrogenase kinase isoenzyme 4
    • Pyruvate dehydrogenase kinase isoform 4
    • Pyruvate dehydrogenase kinase isozyme 4
    • Pyruvate dehydrogenase kinase isozyme 4 mitochondrial
    see all
  • Function
    Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
  • Tissue specificity
    Ubiquitous; highest levels of expression in heart and skeletal muscle.
  • Sequence similarities
    Belongs to the PDK/BCKDK protein kinase family.
    Contains 1 histidine kinase domain.
  • Cellular localization
    Mitochondrion matrix.
  • Information by UniProt

Images

  • The specific activity of ab125560 was determined to be 20 nmol/min/mg.
  • SDS PAGE analysis of ab125560 (PDK4 protein).

References

ab125560 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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