Recombinant human PDP2 protein (ab110358)

Overview

  • Product name
    Recombinant human PDP2 protein
  • Protein length
    Full length protein

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Amino acids
      67 to 529
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab110358 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Sandwich ELISA

    Functional Studies

  • Purity
    > 90 % Purified via His tag.

  • Form
    Liquid
  • Additional notes

    PDH Kinases (PDKs) deactivate PDH by reversible phosphorylation. In humans and rodents, 4 PDK's are present in an organ-dependent manner. In all cases, PDK's are physically part of the PDH complex (binding via lipoyl domain 2 of E2), and they phosphorylate the E1 subunit at 3 specific serine residues. PDH phosphatases remove the phosphorylation and restore activity of PDH.

    Product was previously marketed under the MitoSciences sub-brand.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.14% Sodium phosphate, 1.46% Sodium chloride, 30% Glycerol, 0.08% DTT, 0.02% EDTA, 0.05% Triton-X-100

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 2
    • KIAA1348
    • mitochondrial
    • PDP 2
    • Pdp2
    • PDP2_HUMAN
    • PDPC 2
    • PPM2B
    • PPM2C2
    • Protein phosphatase 2C magnesium dependent catalytic subunit 2
    • Protein phosphatase Mg2+/Mn2+ dependent 2B
    • Pyruvate dehydrogenase phosphatase 2
    • Pyruvate dehydrogenase phosphatase catalytic subunit 2
    • pyruvate dehydrogenase phosphatase isoenzyme 2
    • Pyruvate dehydrogenase phosphatase, catalytic subunit 2
    see all
  • Function
    Catalyzes the dephosphorylation and concomitant reactivation of the alpha subunit of the E1 component of the pyruvate dehydrogenase complex.
  • Sequence similarities
    Belongs to the PP2C family.
  • Cellular localization
    Mitochondrion matrix.
  • Information by UniProt

References

ab110358 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

We advise use of PBS for homogenization of muscle tissue. We have not tested the use of this kit with fat tissue. If you have a favorite homogenization buffer, we would advice to prepare the homogenates of both tissues in both buffers in parallel , run...

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Thank you for your enquiry.

I can confirm that these proteins have been used in functional assays in the following publications using Pyruvate-dehydrogenase-PDH-Enzyme-Activity-Microplate-Assay-Kit-ab109902:

http://www.abcam.com/i...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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