Recombinant human PDP2 protein (ab110358)

Overview

  • Product name
    Recombinant human PDP2 protein
  • Protein length
    Full length protein

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Amino acids
      67 to 529
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab110358 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Sandwich ELISA

    Functional Studies

  • Purity
    > 90 % Purified via His tag.

  • Form
    Liquid
  • Additional notes

    PDH Kinases (PDKs) deactivate PDH by reversible phosphorylation. In humans and rodents, 4 PDK's are present in an organ-dependent manner. In all cases, PDK's are physically part of the PDH complex (binding via lipoyl domain 2 of E2), and they phosphorylate the E1 subunit at 3 specific serine residues. PDH phosphatases remove the phosphorylation and restore activity of PDH.

    Product was previously marketed under the MitoSciences sub-brand.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.14% Sodium phosphate, 1.46% Sodium chloride, 30% Glycerol, 0.08% DTT, 0.02% EDTA, 0.05% Triton-X-100

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 2
    • KIAA1348
    • mitochondrial
    • PDP 2
    • Pdp2
    • PDP2_HUMAN
    • PDPC 2
    • PPM2B
    • PPM2C2
    • Protein phosphatase 2C magnesium dependent catalytic subunit 2
    • Protein phosphatase Mg2+/Mn2+ dependent 2B
    • Pyruvate dehydrogenase phosphatase 2
    • Pyruvate dehydrogenase phosphatase catalytic subunit 2
    • pyruvate dehydrogenase phosphatase isoenzyme 2
    • Pyruvate dehydrogenase phosphatase, catalytic subunit 2
    see all
  • Function
    Catalyzes the dephosphorylation and concomitant reactivation of the alpha subunit of the E1 component of the pyruvate dehydrogenase complex.
  • Sequence similarities
    Belongs to the PP2C family.
  • Cellular localization
    Mitochondrion matrix.
  • Information by UniProt

References for Recombinant human PDP2 protein (ab110358)

ab110358 has not yet been referenced specifically in any publications.

Product Wall

We advise use of PBS for homogenization of muscle tissue. We have not tested the use of this kit with fat tissue. If you have a favorite homogenization buffer, we would advice to prepare the homogenates of both tissues in both buffers in parallel , run...

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Thank you for your enquiry.

I can confirm that these proteins have been used in functional assays in the following publications using Pyruvate-dehydrogenase-PDH-Enzyme-Activity-Microplate-Assay-Kit-ab109902:

http://www.abcam.com/i...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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